Combined information from Raman spectroscopy and optical coherence tomography for enhanced diagnostic accuracy in tissue discrimination

Praveen C. Ashok, Bavishna B. Praveen, Nicola Bellini, Andrew Riches, Kishan Dholakia, C. Simon Herrington

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical spectroscopy and imaging methods have proved to have potential to discriminate between normal and abnormal tissue types through minimally invasive procedures. Raman spectroscopy and Optical Coherence Tomography (OCT) provides chemical and morphological information of tissues respectively, which are complementary to each other. When used individually they might not be able to obtain high enough sensitivity and specificity that is clinically relevant. In this study we combined Raman spectroscopy information with information obtained from OCT to enhance the sensitivity and specificity in discriminating between Colonic Adenocarcinoma from Normal Colon. OCT being an imaging technique, the information from this technique is conventionally analyzed qualitatively. To combine with Raman spectroscopy information, it was essential to quantify the morphological information obtained from OCT. Texture analysis was used to extract information from OCT images, which in-turn was combined with the information obtained from Raman spectroscopy. The sensitivity and specificity of the classifier was estimated using leave one out cross validation (LOOCV) method where support vector machine (SVM) was used for binary classification of the tissues. The sensitivity obtained using Raman spectroscopy and OCT individually was 89% and 78% respectively and the specificity was 77% and 74% respectively. Combining the information derived using the two techniques increased both sensitivity and specificity to 94% demonstrating that combining complementary optical information enhances diagnostic accuracy. These results demonstrate that a multimodal approach using Raman-OCT would be able to enhance the diagnostic accuracy for identifying normal and cancerous tissue types.

Original languageEnglish
Title of host publicationBiomedical Vibrational Spectroscopy VI
Subtitle of host publicationAdvances in Research and Industry
PublisherSPIE
ISBN (Print)9780819498526
DOIs
Publication statusPublished - 2014 Jan 1
EventBiomedical Vibrational Spectroscopy VI: Advances in Research and Industry - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 2

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8939
ISSN (Print)1605-7422

Conference

ConferenceBiomedical Vibrational Spectroscopy VI: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period14/2/114/2/2

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Ashok, P. C., Praveen, B. B., Bellini, N., Riches, A., Dholakia, K., & Herrington, C. S. (2014). Combined information from Raman spectroscopy and optical coherence tomography for enhanced diagnostic accuracy in tissue discrimination. In Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry [2039855] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8939). SPIE. https://doi.org/10.1117/12.2039855