Commensal-derived metabolites govern Vibrio cholerae pathogenesis in host intestine

Jin Sun You, Ji Hyun Yong, Gwang Hee Kim, Sungmin Moon, Ki Taek Nam, Ji Hwan Ryu, Mi Young Yoon, Sang Sun Yoon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Recent evidence suggests that the commensal microbes act as a barrier against invading pathogens and enteric infections are the consequences of multi-layered interactions among commensals, pathogens, and the host intestinal tissue. However, it remains unclear how perturbations of the gut microbiota compromise host infection resistance, especially through changes at species and metabolite levels. Results: Here, we illustrate how Bacteroides vulgatus, a dominant species of the Bacteroidetes phylum in mouse intestine, suppresses infection by Vibrio cholerae, an important human pathogen. Clindamycin (CL) is an antibiotic that selectively kills anaerobic bacteria, and accordingly Bacteroidetes are completely eradicated from CL-treated mouse intestines. The Bacteroidetes-depleted adult mice developed severe cholera-like symptoms, when infected with V. cholerae. Germ-free mice mono-associated with B. vulgatus became resistant to V. cholerae infection. Levels of V. cholerae growth-inhibitory metabolites including short-chain fatty acids plummeted upon CL treatment, while levels of compounds that enhance V. cholerae proliferation were elevated. Furthermore, the intestinal colonization process of V. cholerae was well-simulated in CL-treated adult mice. Conclusions: Overall, we provide insights into how a symbiotic microbe and a pathogenic intruder interact inside host intestine. We identified B. vulgatus as an indigenous microbial species that can suppress intestinal infection. Our results also demonstrate that commensal-derived metabolites are a critical determinant for host resistance against V. cholerae infection, and that CL pretreatment of adult mice generates a simple yet useful model of cholera infection.

Original languageEnglish
Article number132
JournalMicrobiome
Volume7
Issue number1
DOIs
Publication statusPublished - 2019 Sep 14

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation (NRF) of Korea, which is funded by the Korean Government, 2017R1A2A2A05019987 and 2017M3A9F3041233, 2019R1A6A1A03032869, and 2017R1A1A1A05001200. This work was also supported by Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (918003041SB010).

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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