Comparative analyses of plasma amyloid-b levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system

YoungSoo Kim, Yong Kyoung Yoo, Hye Yun Kim, Jee Hoon Roh, Jinsik Kim, Seungyeop Baek, Jinny Claire Lee, Hye Jin Kim, Myung Sic Chae, Dahye Jeong, Dongsung Park, Sejin Lee, Ho Chung Jang, Kyeonghwan Kim, Jeong Hoon Lee, Byung Hyun Byun, Su Yeon Park, Jeong Ho Ha, Kyo Chul Lee, Won Woo ChoJae Seung Kim, Jae Young Koh, Sang Moo Lim, Kyo Seon Hwang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Detection of amyloid-b (Ab) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Ab is deemed a less invasive and more accessible hallmark of AD, as Ab can penetrate blood-brain barriers. However, correlations between biofluidic Ab concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of Ab in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated Ab into monomers to enhance quantification accuracy. Subsequently, Ab levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter- and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma Ab levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.

Original languageEnglish
Article numbereaav1388
JournalScience Advances
Volume5
Issue number4
DOIs
Publication statusPublished - 2019 Jan 1

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sensors
blood-brain barrier
cognition
acids
progressions
blood
monomers
selectivity
sensitivity
molecules

All Science Journal Classification (ASJC) codes

  • General
  • Physics and Astronomy (miscellaneous)

Cite this

Kim, YoungSoo ; Yoo, Yong Kyoung ; Kim, Hye Yun ; Roh, Jee Hoon ; Kim, Jinsik ; Baek, Seungyeop ; Lee, Jinny Claire ; Kim, Hye Jin ; Chae, Myung Sic ; Jeong, Dahye ; Park, Dongsung ; Lee, Sejin ; Jang, Ho Chung ; Kim, Kyeonghwan ; Lee, Jeong Hoon ; Byun, Byung Hyun ; Park, Su Yeon ; Ha, Jeong Ho ; Lee, Kyo Chul ; Cho, Won Woo ; Kim, Jae Seung ; Koh, Jae Young ; Lim, Sang Moo ; Hwang, Kyo Seon. / Comparative analyses of plasma amyloid-b levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system. In: Science Advances. 2019 ; Vol. 5, No. 4.
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abstract = "Detection of amyloid-b (Ab) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Ab is deemed a less invasive and more accessible hallmark of AD, as Ab can penetrate blood-brain barriers. However, correlations between biofluidic Ab concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of Ab in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated Ab into monomers to enhance quantification accuracy. Subsequently, Ab levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter- and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma Ab levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90{\%}.",
author = "YoungSoo Kim and Yoo, {Yong Kyoung} and Kim, {Hye Yun} and Roh, {Jee Hoon} and Jinsik Kim and Seungyeop Baek and Lee, {Jinny Claire} and Kim, {Hye Jin} and Chae, {Myung Sic} and Dahye Jeong and Dongsung Park and Sejin Lee and Jang, {Ho Chung} and Kyeonghwan Kim and Lee, {Jeong Hoon} and Byun, {Byung Hyun} and Park, {Su Yeon} and Ha, {Jeong Ho} and Lee, {Kyo Chul} and Cho, {Won Woo} and Kim, {Jae Seung} and Koh, {Jae Young} and Lim, {Sang Moo} and Hwang, {Kyo Seon}",
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Kim, Y, Yoo, YK, Kim, HY, Roh, JH, Kim, J, Baek, S, Lee, JC, Kim, HJ, Chae, MS, Jeong, D, Park, D, Lee, S, Jang, HC, Kim, K, Lee, JH, Byun, BH, Park, SY, Ha, JH, Lee, KC, Cho, WW, Kim, JS, Koh, JY, Lim, SM & Hwang, KS 2019, 'Comparative analyses of plasma amyloid-b levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system', Science Advances, vol. 5, no. 4, eaav1388. https://doi.org/10.1126/sciadv.aav1388

Comparative analyses of plasma amyloid-b levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system. / Kim, YoungSoo; Yoo, Yong Kyoung; Kim, Hye Yun; Roh, Jee Hoon; Kim, Jinsik; Baek, Seungyeop; Lee, Jinny Claire; Kim, Hye Jin; Chae, Myung Sic; Jeong, Dahye; Park, Dongsung; Lee, Sejin; Jang, Ho Chung; Kim, Kyeonghwan; Lee, Jeong Hoon; Byun, Byung Hyun; Park, Su Yeon; Ha, Jeong Ho; Lee, Kyo Chul; Cho, Won Woo; Kim, Jae Seung; Koh, Jae Young; Lim, Sang Moo; Hwang, Kyo Seon.

In: Science Advances, Vol. 5, No. 4, eaav1388, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Kim, YoungSoo

AU - Yoo, Yong Kyoung

AU - Kim, Hye Yun

AU - Roh, Jee Hoon

AU - Kim, Jinsik

AU - Baek, Seungyeop

AU - Lee, Jinny Claire

AU - Kim, Hye Jin

AU - Chae, Myung Sic

AU - Jeong, Dahye

AU - Park, Dongsung

AU - Lee, Sejin

AU - Jang, Ho Chung

AU - Kim, Kyeonghwan

AU - Lee, Jeong Hoon

AU - Byun, Byung Hyun

AU - Park, Su Yeon

AU - Ha, Jeong Ho

AU - Lee, Kyo Chul

AU - Cho, Won Woo

AU - Kim, Jae Seung

AU - Koh, Jae Young

AU - Lim, Sang Moo

AU - Hwang, Kyo Seon

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Detection of amyloid-b (Ab) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Ab is deemed a less invasive and more accessible hallmark of AD, as Ab can penetrate blood-brain barriers. However, correlations between biofluidic Ab concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of Ab in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated Ab into monomers to enhance quantification accuracy. Subsequently, Ab levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter- and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma Ab levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.

AB - Detection of amyloid-b (Ab) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Ab is deemed a less invasive and more accessible hallmark of AD, as Ab can penetrate blood-brain barriers. However, correlations between biofluidic Ab concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of Ab in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated Ab into monomers to enhance quantification accuracy. Subsequently, Ab levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter- and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma Ab levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.

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