Comparative analyses of plasma amyloid-b levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system

Young Soo Kim, Yong Kyoung Yoo, Hye Yun Kim, Jee Hoon Roh, Jinsik Kim, Seungyeop Baek, Jinny Claire Lee, Hye Jin Kim, Myung Sic Chae, Dahye Jeong, Dongsung Park, Sejin Lee, Ho Chung Jang, Kyeonghwan Kim, Jeong Hoon Lee, Byung Hyun Byun, Su Yeon Park, Jeong Ho Ha, Kyo Chul Lee, Won Woo ChoJae Seung Kim, Jae Young Koh, Sang Moo Lim, Kyo Seon Hwang

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Abstract

Detection of amyloid-b (Ab) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Ab is deemed a less invasive and more accessible hallmark of AD, as Ab can penetrate blood-brain barriers. However, correlations between biofluidic Ab concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of Ab in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated Ab into monomers to enhance quantification accuracy. Subsequently, Ab levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter- and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma Ab levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.

Original languageEnglish
Article numbereaav1388
JournalScience Advances
Volume5
Issue number4
DOIs
Publication statusPublished - 2019 Jan 1

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All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)
  • General

Cite this

Kim, Y. S., Yoo, Y. K., Kim, H. Y., Roh, J. H., Kim, J., Baek, S., Lee, J. C., Kim, H. J., Chae, M. S., Jeong, D., Park, D., Lee, S., Jang, H. C., Kim, K., Lee, J. H., Byun, B. H., Park, S. Y., Ha, J. H., Lee, K. C., ... Hwang, K. S. (2019). Comparative analyses of plasma amyloid-b levels in heterogeneous and monomerized states by interdigitated microelectrode sensor system. Science Advances, 5(4), [eaav1388]. https://doi.org/10.1126/sciadv.aav1388