Abstract
The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2′-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluoresence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localize with LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells.
| Original language | English |
|---|---|
| Pages (from-to) | 47-53 |
| Number of pages | 7 |
| Journal | Cell and Tissue Research |
| Volume | 334 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Oct 1 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Histology
- Cell Biology
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Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland. / Kim, Yeun Jung; Kwon, Hyuk Jae; Shinozaki, Naoshi; Hashimoto, Sadamitsu; Shimono, Masaki; Cho, Sung Won; Jung, Han Sung.
In: Cell and Tissue Research, Vol. 334, No. 1, 01.10.2008, p. 47-53.Research output: Contribution to journal › Article
TY - JOUR
T1 - Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland
AU - Kim, Yeun Jung
AU - Kwon, Hyuk Jae
AU - Shinozaki, Naoshi
AU - Hashimoto, Sadamitsu
AU - Shimono, Masaki
AU - Cho, Sung Won
AU - Jung, Han Sung
PY - 2008/10/1
Y1 - 2008/10/1
N2 - The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2′-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluoresence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localize with LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells.
AB - The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2′-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluoresence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localize with LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells.
UR - http://www.scopus.com/inward/record.url?scp=51749116278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51749116278&partnerID=8YFLogxK
U2 - 10.1007/s00441-008-0667-8
DO - 10.1007/s00441-008-0667-8
M3 - Article
C2 - 18688650
AN - SCOPUS:51749116278
VL - 334
SP - 47
EP - 53
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 1
ER -