Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10

John S. Spencer, Hee Jin Kim, Angela M. Marques, Mercedes Gonzalez-Juarerro, Monica C.B.S. Lima, Varalakshmi D. Vissa, Richard W. Truman, Maria Laura Gennaro, Sangnae Cho, Stewart T. Cole, Patrick J. Brennan

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Culture filtrate protein 10 (CFP-10) from Mycobacterium tuberculosis is a well-characterized immunodominant 10-kDa protein antigen known to elicit a very potent early gamma interferon response in T cells from M. tuberculosis-infected mice and humans. The sequence of the Mycobacterium leprae homologue of CFP-10 shows only 40% identity (60% homology) at the protein level with M. tuberculosis CFP-10 and thus has the potential for development as a T- or B-cell reactive antigen for specific diagnosis of leprosy. Antisera raised in mice or rabbits against recombinant M. leprae and M. tuberculosis CFP-10 proteins reacted only with homologous peptides from arrays of overlapping synthetic peptides, indicating that there was no detectable cross-reactivity at the antibody level. Sera from leprosy and tuberculosis patients were also specific for the homologous protein or peptides and showed distinct patterns of recognition for either M. leprae or M. tuberculosis CFP-10 peptides. At the cellular level, only 2 of 45 mouse T-cell hybridomas raised against either M. leprae or M. tuberculosis CFP-10 displayed a cross-reactive response against the N-terminal heterologous CFP-10 peptide, the region that exhibits the highest level of identity in the two proteins; however, the majority of peptide epitopes recognized by mouse T-cell hybridomas specific for each protein did not cross-react with heterologous peptides. Coupled with the human serology data, these results raise the possibility that peptides that could be used to differentiate infections caused by these two related microorganisms could be developed. Immunohistochemical staining of sections of M. leprae-infected nude mouse footpads resulted in strongly positive staining in macrophages and dendritic cells, as well as weaker staining in extracellular areas, suggesting that M. leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein.

Original languageEnglish
Pages (from-to)3161-3170
Number of pages10
JournalInfection and Immunity
Volume72
Issue number6
DOIs
Publication statusPublished - 2004 Jun 1

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B-Lymphocyte Epitopes
Mycobacterium leprae
T-Lymphocyte Epitopes
Mycobacterium tuberculosis
Proteins
Peptides
Hybridomas
Leprosy
Staining and Labeling
T-Lymphocytes
Antigens
Serology
Mycobacterium
Nude Mice
Dendritic Cells
Interferon-gamma

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Spencer, J. S., Kim, H. J., Marques, A. M., Gonzalez-Juarerro, M., Lima, M. C. B. S., Vissa, V. D., ... Brennan, P. J. (2004). Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10. Infection and Immunity, 72(6), 3161-3170. https://doi.org/10.1128/IAI.72.6.3161-3170.2004
Spencer, John S. ; Kim, Hee Jin ; Marques, Angela M. ; Gonzalez-Juarerro, Mercedes ; Lima, Monica C.B.S. ; Vissa, Varalakshmi D. ; Truman, Richard W. ; Gennaro, Maria Laura ; Cho, Sangnae ; Cole, Stewart T. ; Brennan, Patrick J. / Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10. In: Infection and Immunity. 2004 ; Vol. 72, No. 6. pp. 3161-3170.
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Spencer, JS, Kim, HJ, Marques, AM, Gonzalez-Juarerro, M, Lima, MCBS, Vissa, VD, Truman, RW, Gennaro, ML, Cho, S, Cole, ST & Brennan, PJ 2004, 'Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10', Infection and Immunity, vol. 72, no. 6, pp. 3161-3170. https://doi.org/10.1128/IAI.72.6.3161-3170.2004

Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10. / Spencer, John S.; Kim, Hee Jin; Marques, Angela M.; Gonzalez-Juarerro, Mercedes; Lima, Monica C.B.S.; Vissa, Varalakshmi D.; Truman, Richard W.; Gennaro, Maria Laura; Cho, Sangnae; Cole, Stewart T.; Brennan, Patrick J.

In: Infection and Immunity, Vol. 72, No. 6, 01.06.2004, p. 3161-3170.

Research output: Contribution to journalArticle

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T1 - Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10

AU - Spencer, John S.

AU - Kim, Hee Jin

AU - Marques, Angela M.

AU - Gonzalez-Juarerro, Mercedes

AU - Lima, Monica C.B.S.

AU - Vissa, Varalakshmi D.

AU - Truman, Richard W.

AU - Gennaro, Maria Laura

AU - Cho, Sangnae

AU - Cole, Stewart T.

AU - Brennan, Patrick J.

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