Comparative analysis of tagatose productivity of immobilized L-arabinose isomerase expressed in Escherichia coli and Bacillus subtilis

Jina Cheon, Bo Kim Seong, Won Park Seong, Kwon Han Jong, Pil Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although arabinose isomerase (E.C. 5.3.1.4), a commercial enzyme for edible tagatose bioconversion, can be expressed in an Escherichia coli system, this expression system might leave noxious by-products in food. To develop an eligible tagatose bioconversion with food-safe system, we compared the tagatose production activity of immobilized arabinose isomerase expressed in Bacillus subtilis (a host generally recognized as safe) with that of the enzyme expressed in E. coli. A 48% increase in tagatose production (4.3 g tagatose/L at 69.4 mg/L·hr) was found using the B. subtilis-expressed immobilized enzyme system, compared to the E. coli-expressed enzyme system (2.9 g tagatose/L). The increased productivity with safety of the B. subtilis-expressed arabinose isomerase suggests that it is a more eligible candidate for commercial tagatose production.

Original languageEnglish
Pages (from-to)655-658
Number of pages4
JournalFood Science and Biotechnology
Volume17
Issue number3
Publication statusPublished - 2008 Aug 22

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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