The current study comparatively evaluated the effects of silver nanoparticles (AgNPs) on fermentative bio hydrogen (bio-H2) production by Clostridium beijerinckii (KTCC1737). Green synthesized AgNPs were fabricated using henna (Lawsonia inermis) and DU-C2 actinomycete in batch using different AgNPs concentrations (0, 40, 80, and 120 μg/mL). The overall results revealed that after 72 hours of AgNPs incubation with C beijerinckii, the henna mediated AgNP synthesis increased the hydrogen production rate with a hydrogen yield of 1.71 mol H2/mol glucose added. However, the DU-C2-mediated AgNps synthesis inhibited bio-H2 production during the batch H2 fermentation. Performed live/dead staining showed AgNPs effect on the bacterial culture in batch fermentation. The results indicated that the henna mediated AgNPs enhanced the bacterial cell population, meanwhile, in case of DU-C2 AgNPs, less bacterial population occurred. Thus, the present study provides a clear overview on the application of metallic NPs as H2 production catalysts to further improve bio-H2 production.
Bibliographical noteFunding Information:
National Research Foundation of Korea, Grant/Award Numbers: NRF‐2020R1A2B5B02001757, NRF‐2019M3E6A1103839 Funding information
The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (Ministry of Science and ICT) (Nos. NRF‐2019M3E6A1103839 and NRF‐2020R1A2B5B02001757). Moreover, we thank Prof. Jung Won Yoon, Daegu University, South Korea, for his technical support on our manuscript.
© 2020 John Wiley & Sons Ltd
All Science Journal Classification (ASJC) codes
- Renewable Energy, Sustainability and the Environment
- Nuclear Energy and Engineering
- Fuel Technology
- Energy Engineering and Power Technology