Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans

Hyo Seol Lee, Jongeun Lee, Seong Oh Kim, JeSeon Song, Jae Ho Lee, Syng Ill Lee, Hansung Jung, Byung Jai Choi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), while in the PDL it was the tumor-suppressor gene WIF1. Genes related to bone development and remodeling (STMN2, IBSP, BMP8A, BGLAP, ACP5, OPN, BMP3, and TM7SF4) and wound healing (IL1, IL8, MMP3, and MMP9) were also more strongly expressed in the PDL than in the dental follicle. In selected genes, a comparison among cDNA microarray, real-time reverse-transcription polymerase chain reaction, and immunohistochemical staining confirmed similar relative gene expressions. The gene-expression profiles presented here identify candidate genes that may enable differentiation between the dental follicle and PDL.

Original languageEnglish
Article numbere84201
JournalPloS one
Volume8
Issue number12
DOIs
Publication statusPublished - 2013 Dec 23

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Dental Sac
Periodontal Ligament
Ligaments
ligaments
Gene expression
teeth
Genes
Gene Expression
gene expression
Microarrays
Bone Remodeling
Bone Development
skeletal development
Oligonucleotide Array Sequence Analysis
Transcriptome
genes
Bone
Complementary DNA
Polymerase chain reaction
Chemotaxis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lee, Hyo Seol ; Lee, Jongeun ; Kim, Seong Oh ; Song, JeSeon ; Lee, Jae Ho ; Lee, Syng Ill ; Jung, Hansung ; Choi, Byung Jai. / Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. In: PloS one. 2013 ; Vol. 8, No. 12.
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abstract = "The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), while in the PDL it was the tumor-suppressor gene WIF1. Genes related to bone development and remodeling (STMN2, IBSP, BMP8A, BGLAP, ACP5, OPN, BMP3, and TM7SF4) and wound healing (IL1, IL8, MMP3, and MMP9) were also more strongly expressed in the PDL than in the dental follicle. In selected genes, a comparison among cDNA microarray, real-time reverse-transcription polymerase chain reaction, and immunohistochemical staining confirmed similar relative gene expressions. The gene-expression profiles presented here identify candidate genes that may enable differentiation between the dental follicle and PDL.",
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Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. / Lee, Hyo Seol; Lee, Jongeun; Kim, Seong Oh; Song, JeSeon; Lee, Jae Ho; Lee, Syng Ill; Jung, Hansung; Choi, Byung Jai.

In: PloS one, Vol. 8, No. 12, e84201, 23.12.2013.

Research output: Contribution to journalArticle

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