Comparative Gene Expression Analysis of the Human Periodontal Ligament in Deciduous and Permanent Teeth

Je Seon Song, Dong Hwan Hwang, Seong Oh Kim, Mijeong Jeon, Byung Jai Choi, Han Sung Jung, Seok Jun Moon, Wonse Park, Hyung Jun Choi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

There are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription-polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.

Original languageEnglish
Article numbere61231
JournalPloS one
Volume8
Issue number4
DOIs
Publication statusPublished - 2013 Apr 8

Fingerprint

Periodontal Ligament
Deciduous Tooth
Ligaments
ligaments
Gene expression
teeth
Tissue
Gene Expression
gene expression
Genes
genes
tissues
Antigen-antibody reactions
Degradation
degradation
Polymerase chain reaction
Bicuspid
Transcription
Microarray Analysis
Microarrays

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Song, Je Seon ; Hwang, Dong Hwan ; Kim, Seong Oh ; Jeon, Mijeong ; Choi, Byung Jai ; Jung, Han Sung ; Moon, Seok Jun ; Park, Wonse ; Choi, Hyung Jun. / Comparative Gene Expression Analysis of the Human Periodontal Ligament in Deciduous and Permanent Teeth. In: PloS one. 2013 ; Vol. 8, No. 4.
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abstract = "There are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription-polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.",
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Comparative Gene Expression Analysis of the Human Periodontal Ligament in Deciduous and Permanent Teeth. / Song, Je Seon; Hwang, Dong Hwan; Kim, Seong Oh; Jeon, Mijeong; Choi, Byung Jai; Jung, Han Sung; Moon, Seok Jun; Park, Wonse; Choi, Hyung Jun.

In: PloS one, Vol. 8, No. 4, e61231, 08.04.2013.

Research output: Contribution to journalArticle

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