Comparative microbiome analysis of three species of laboratory-reared periplaneta cockroaches

Seogwon Lee, Ju Yeong Kim, Myung Hee Yi, In Yong Lee, Won Ja Lee, Hye Su Moon, Dongeun Yong, Tai Soon Yong

Research output: Contribution to journalArticlepeer-review

Abstract

Cockroaches inhabit various habitats, which will influence their microbiome. Although the microbiome can be influenced by the diet and environmental factors, it can also differ between species. Therefore, we conducted 16S rDNAtargeted high-throughput sequencing to evaluate the overall bacterial composition of the microbiomes of 3 cockroach species, Periplaneta americana, P. japonica, and P. fuliginosa, raised in laboratory for several generations under the same conditions. The experiments were conducted using male adult cockroaches. The number of operational taxonomic units (OTUs) was not significantly different among the 3 species. With regard to the Shannon and Pielou indexes, higher microbiome values were noted in P. americana than in P. japonica and P. fuliginosa. Microbiome composition was also evaluated, with endosymbionts accounting for over half of all OTUs in P. japonica and P. fuliginosa. Beta diversity analysis further showed that P. japonica and P. fuliginosa had similar microbiome composition, which differed from that of P. americana. However, we also identified that P. japonica and P. fuliginosa host distinct OTUs. Thus, although microbiome compositions may vary based on multiple conditions, it is possible to identify distinct microbiome compositions among different Periplaneta cockroach species, even when the individuals are reared under the same conditions.

Original languageEnglish
Pages (from-to)537-542
Number of pages6
JournalKorean Journal of Parasitology
Volume58
Issue number5
DOIs
Publication statusPublished - 2020 Oct

Bibliographical note

Funding Information:
This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST; numbers NRF-2019R1A2B5B01069843 and 2020R1I1A2074562).

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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