Comparative proteomic analysis of advanced serous epithelial ovarian carcinoma: Possible predictors of chemoresistant disease

Sang Wun Kim, Sunghoon Kim, Eun Ji Nam, Yong Wook Jeong, San Hui Lee, Ji Heum Paek, Jae Hoon Kim, Jae Wook Kim, Young Tae Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To identify specific proteins associated with chemotherapeutic responses, we analyzed protein expression patterns in stage IIIc primary serous epithelial ovarian cancer tissues displaying differential responses to first-line postoperative adjuvant chemotherapy. The expression profiles of five chemoresistant tissues [progression-free survival (PFS) ≤12 months] and five chemosensitive tissues (PFS ≥48 months) were analyzed with 2D electrophoresis, and the spot intensities of differentially expressed proteins were quantified. To validate these proteins as markers for chemoresistant disease, we analyzed tissues from an additional 17 patients. All the patients were allocated to the over- or underexpressing group according to protein spot intensity, and survival analysis was performed. In chemoresistant tissues, four proteins (thioredoxin domain containing four, similar to RIKEN cDNA 1700016G05, tubulin α 1A chain, and the pyruvate dehydrogenase E1-β subunit precursor) were overexpressed, and seven proteins [keratin 1, vitamin D-binding protein, creatine kinase B, annexin V, SH3-containing guanine nucleotide exchange factor (SGEF), tryptophan-aspartate repeat protein-1 (WDR 1), and WDR 1 isoform 1] were underexpressed. The underexpression of keratin 1, creatine kinase B, annexin V, SGEF, WDR1, and WDR1 isoform 1 were significantly correlated with poor overall survival. A combination of keratin 1 and SGEF showed the highest sensitivity of 0.800, specificity of 0.917, PPV of 0.800, and NPV of 0.917 in predicting chemoresistant disease. These proteins may be useful as predictive markers of chemoresistant disease. However, further analyses in large-scale should be performed before they can be considered reliable predictive markers of chemoresistant disease.

Original languageEnglish
Pages (from-to)281-292
Number of pages12
JournalOMICS A Journal of Integrative Biology
Volume15
Issue number5
DOIs
Publication statusPublished - 2011 May 1

Fingerprint

Proteomics
Carcinoma
Keratin-1
Guanine Nucleotide Exchange Factors
Proteins
Tissue
Annexin A5
Creatine Kinase
Disease-Free Survival
Pyruvate Dehydrogenase (Lipoamide)
Protein Isoforms
Vitamin D-Binding Protein
Proto-Oncogene Proteins c-akt
Thioredoxins
Chemotherapy
Tubulin
Adjuvant Chemotherapy
Survival Analysis
Aspartic Acid
Tryptophan

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Kim, Sang Wun ; Kim, Sunghoon ; Nam, Eun Ji ; Jeong, Yong Wook ; Lee, San Hui ; Paek, Ji Heum ; Kim, Jae Hoon ; Kim, Jae Wook ; Kim, Young Tae. / Comparative proteomic analysis of advanced serous epithelial ovarian carcinoma : Possible predictors of chemoresistant disease. In: OMICS A Journal of Integrative Biology. 2011 ; Vol. 15, No. 5. pp. 281-292.
@article{4d49bca06ef94b5daccf0518dd43faa2,
title = "Comparative proteomic analysis of advanced serous epithelial ovarian carcinoma: Possible predictors of chemoresistant disease",
abstract = "To identify specific proteins associated with chemotherapeutic responses, we analyzed protein expression patterns in stage IIIc primary serous epithelial ovarian cancer tissues displaying differential responses to first-line postoperative adjuvant chemotherapy. The expression profiles of five chemoresistant tissues [progression-free survival (PFS) ≤12 months] and five chemosensitive tissues (PFS ≥48 months) were analyzed with 2D electrophoresis, and the spot intensities of differentially expressed proteins were quantified. To validate these proteins as markers for chemoresistant disease, we analyzed tissues from an additional 17 patients. All the patients were allocated to the over- or underexpressing group according to protein spot intensity, and survival analysis was performed. In chemoresistant tissues, four proteins (thioredoxin domain containing four, similar to RIKEN cDNA 1700016G05, tubulin α 1A chain, and the pyruvate dehydrogenase E1-β subunit precursor) were overexpressed, and seven proteins [keratin 1, vitamin D-binding protein, creatine kinase B, annexin V, SH3-containing guanine nucleotide exchange factor (SGEF), tryptophan-aspartate repeat protein-1 (WDR 1), and WDR 1 isoform 1] were underexpressed. The underexpression of keratin 1, creatine kinase B, annexin V, SGEF, WDR1, and WDR1 isoform 1 were significantly correlated with poor overall survival. A combination of keratin 1 and SGEF showed the highest sensitivity of 0.800, specificity of 0.917, PPV of 0.800, and NPV of 0.917 in predicting chemoresistant disease. These proteins may be useful as predictive markers of chemoresistant disease. However, further analyses in large-scale should be performed before they can be considered reliable predictive markers of chemoresistant disease.",
author = "Kim, {Sang Wun} and Sunghoon Kim and Nam, {Eun Ji} and Jeong, {Yong Wook} and Lee, {San Hui} and Paek, {Ji Heum} and Kim, {Jae Hoon} and Kim, {Jae Wook} and Kim, {Young Tae}",
year = "2011",
month = "5",
day = "1",
doi = "10.1089/omi.2010.0012",
language = "English",
volume = "15",
pages = "281--292",
journal = "OMICS A Journal of Integrative Biology",
issn = "1536-2310",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

Comparative proteomic analysis of advanced serous epithelial ovarian carcinoma : Possible predictors of chemoresistant disease. / Kim, Sang Wun; Kim, Sunghoon; Nam, Eun Ji; Jeong, Yong Wook; Lee, San Hui; Paek, Ji Heum; Kim, Jae Hoon; Kim, Jae Wook; Kim, Young Tae.

In: OMICS A Journal of Integrative Biology, Vol. 15, No. 5, 01.05.2011, p. 281-292.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparative proteomic analysis of advanced serous epithelial ovarian carcinoma

T2 - Possible predictors of chemoresistant disease

AU - Kim, Sang Wun

AU - Kim, Sunghoon

AU - Nam, Eun Ji

AU - Jeong, Yong Wook

AU - Lee, San Hui

AU - Paek, Ji Heum

AU - Kim, Jae Hoon

AU - Kim, Jae Wook

AU - Kim, Young Tae

PY - 2011/5/1

Y1 - 2011/5/1

N2 - To identify specific proteins associated with chemotherapeutic responses, we analyzed protein expression patterns in stage IIIc primary serous epithelial ovarian cancer tissues displaying differential responses to first-line postoperative adjuvant chemotherapy. The expression profiles of five chemoresistant tissues [progression-free survival (PFS) ≤12 months] and five chemosensitive tissues (PFS ≥48 months) were analyzed with 2D electrophoresis, and the spot intensities of differentially expressed proteins were quantified. To validate these proteins as markers for chemoresistant disease, we analyzed tissues from an additional 17 patients. All the patients were allocated to the over- or underexpressing group according to protein spot intensity, and survival analysis was performed. In chemoresistant tissues, four proteins (thioredoxin domain containing four, similar to RIKEN cDNA 1700016G05, tubulin α 1A chain, and the pyruvate dehydrogenase E1-β subunit precursor) were overexpressed, and seven proteins [keratin 1, vitamin D-binding protein, creatine kinase B, annexin V, SH3-containing guanine nucleotide exchange factor (SGEF), tryptophan-aspartate repeat protein-1 (WDR 1), and WDR 1 isoform 1] were underexpressed. The underexpression of keratin 1, creatine kinase B, annexin V, SGEF, WDR1, and WDR1 isoform 1 were significantly correlated with poor overall survival. A combination of keratin 1 and SGEF showed the highest sensitivity of 0.800, specificity of 0.917, PPV of 0.800, and NPV of 0.917 in predicting chemoresistant disease. These proteins may be useful as predictive markers of chemoresistant disease. However, further analyses in large-scale should be performed before they can be considered reliable predictive markers of chemoresistant disease.

AB - To identify specific proteins associated with chemotherapeutic responses, we analyzed protein expression patterns in stage IIIc primary serous epithelial ovarian cancer tissues displaying differential responses to first-line postoperative adjuvant chemotherapy. The expression profiles of five chemoresistant tissues [progression-free survival (PFS) ≤12 months] and five chemosensitive tissues (PFS ≥48 months) were analyzed with 2D electrophoresis, and the spot intensities of differentially expressed proteins were quantified. To validate these proteins as markers for chemoresistant disease, we analyzed tissues from an additional 17 patients. All the patients were allocated to the over- or underexpressing group according to protein spot intensity, and survival analysis was performed. In chemoresistant tissues, four proteins (thioredoxin domain containing four, similar to RIKEN cDNA 1700016G05, tubulin α 1A chain, and the pyruvate dehydrogenase E1-β subunit precursor) were overexpressed, and seven proteins [keratin 1, vitamin D-binding protein, creatine kinase B, annexin V, SH3-containing guanine nucleotide exchange factor (SGEF), tryptophan-aspartate repeat protein-1 (WDR 1), and WDR 1 isoform 1] were underexpressed. The underexpression of keratin 1, creatine kinase B, annexin V, SGEF, WDR1, and WDR1 isoform 1 were significantly correlated with poor overall survival. A combination of keratin 1 and SGEF showed the highest sensitivity of 0.800, specificity of 0.917, PPV of 0.800, and NPV of 0.917 in predicting chemoresistant disease. These proteins may be useful as predictive markers of chemoresistant disease. However, further analyses in large-scale should be performed before they can be considered reliable predictive markers of chemoresistant disease.

UR - http://www.scopus.com/inward/record.url?scp=79956062762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956062762&partnerID=8YFLogxK

U2 - 10.1089/omi.2010.0012

DO - 10.1089/omi.2010.0012

M3 - Article

C2 - 21332407

AN - SCOPUS:79956062762

VL - 15

SP - 281

EP - 292

JO - OMICS A Journal of Integrative Biology

JF - OMICS A Journal of Integrative Biology

SN - 1536-2310

IS - 5

ER -