Comparative proteomics of ovarian epithelial tumors

Hee Jung An, Dong Su Kim, Yong Kyu Park, Sei Kwang Kim, Yoon Pyo Choi, Suki Kang, Boxiao Ding, Nam Hoon Cho

Research output: Contribution to journalArticle

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Abstract

We analyzed 12 ovarian epithelial tumors using 2D PAGE-based comparative proteomics to construct intra- and inter-tumoral distance map trees and to discover surrogate biomarkers indicative of an ovarian tumor. The analysis was performed after laser microdissection of 12 fresh-frozen tissue samples, including 4 serous, 5 mucinous, and 3 endometrioid tumors, with correlation with their histopathological characteristics. Ovarian epithelial tumors and normal tissues showed an apparent separation on the distance map tree. Mucinous carcinomas were closest to the normal group, whereas serous carcinomas were located furthest from the normal group. All mucinous tumors with aggressive histology were separated from the low malignant potential (LMP) group. The benign-looking cysts adjacent to the intraepithelial carcinoma (IEC) showed an expression pattern identical to that of the IEC area. The extent of change on the lineages leading to the mucinous and serous carcinoma was 1.98-fold different. The overall gene expression profiles of serous or endometrioid carcinomas appeared to be less affected by grade or stage than by histologic type. The potential candidate biomarkers screened in ovarian tumors and found to be significantly up-regulated in comparison to normal tissues were as follows: NM23, annexin-1, protein phosphatase-1, ferritin light chain, proteasome a-6, and NAGK (N-acetyl glucosamine kinase). In conclusion, ovarian mucinous tumors are distinct from other ovarian epithelial tumors. LMP mucinous tumors showing histologically aggressive features belong to mucinous carcinoma on the proteomic basis.

Original languageEnglish
Pages (from-to)1082-1090
Number of pages9
JournalJournal of Proteome Research
Volume5
Issue number5
DOIs
Publication statusPublished - 2006 May 1

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Proteomics
Tumors
Mucinous Adenocarcinoma
Neoplasms
Carcinoma in Situ
Biomarkers
Tissue
Microdissection
Apoferritins
Endometrioid Carcinoma
Annexins
Protein Phosphatase 1
Histology
Glucosamine
Electrophoresis, Gel, Two-Dimensional
Proteasome Endopeptidase Complex
Transcriptome
Gene expression
Cysts
Lasers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

An, H. J., Kim, D. S., Park, Y. K., Kim, S. K., Choi, Y. P., Kang, S., ... Cho, N. H. (2006). Comparative proteomics of ovarian epithelial tumors. Journal of Proteome Research, 5(5), 1082-1090. https://doi.org/10.1021/pr050461p
An, Hee Jung ; Kim, Dong Su ; Park, Yong Kyu ; Kim, Sei Kwang ; Choi, Yoon Pyo ; Kang, Suki ; Ding, Boxiao ; Cho, Nam Hoon. / Comparative proteomics of ovarian epithelial tumors. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 5. pp. 1082-1090.
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An, HJ, Kim, DS, Park, YK, Kim, SK, Choi, YP, Kang, S, Ding, B & Cho, NH 2006, 'Comparative proteomics of ovarian epithelial tumors', Journal of Proteome Research, vol. 5, no. 5, pp. 1082-1090. https://doi.org/10.1021/pr050461p

Comparative proteomics of ovarian epithelial tumors. / An, Hee Jung; Kim, Dong Su; Park, Yong Kyu; Kim, Sei Kwang; Choi, Yoon Pyo; Kang, Suki; Ding, Boxiao; Cho, Nam Hoon.

In: Journal of Proteome Research, Vol. 5, No. 5, 01.05.2006, p. 1082-1090.

Research output: Contribution to journalArticle

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An HJ, Kim DS, Park YK, Kim SK, Choi YP, Kang S et al. Comparative proteomics of ovarian epithelial tumors. Journal of Proteome Research. 2006 May 1;5(5):1082-1090. https://doi.org/10.1021/pr050461p