Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Young Joo Choi, Su Jin Kang, Yang Jee Kim, Yong Beom Lim, Hai Won Chung

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

A safe alternative to the viral system used in gene therapy is a nonviral gene delivery system. Although polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer are among the most promising gene-carrier candidates for efficient nonviral gene delivery, safety concerns regarding their toxicity remain. The aim of this study was to scrutinize the underlying mechanism of the cytotoxicity and genotoxicity of PEI (25 kDa) and PAMAM (G4). To our knowledge, this is the first study to explore the genotoxic effect of polymeric gene carriers. To evaluate cell death by PEI and PAMAM, we performed propidium-iodide staining and lactate-dehydrogenase release assays. The genotoxicity of the polymers was measured by comet assay and cytokinesis-block micronucleus assay. PEI-and PAMAM-treated groups induced both necrotic and apoptotic cell death. In the comet assay and micronuclei formation, significant increases in DNA damage were observed in both treatments. We conclude that PEI and PAMAM dendrimer can induce not only a relatively weak apoptotic and a strong necrotic effect, but also a moderate genotoxic effect.

Original languageEnglish
Pages (from-to)357-366
Number of pages10
JournalDrug and Chemical Toxicology
Volume33
Issue number4
DOIs
Publication statusPublished - 2010 Oct

Bibliographical note

Funding Information:
This work was supported by the Korean Ministry of Education, Science, and Technology through the Brain Korea 21 program (2009).

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Fingerprint Dive into the research topics of 'Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells'. Together they form a unique fingerprint.

Cite this