Comparative Study of hydroxyapatite prepared from seashells and eggshells as a bone graft material

Sang Woon Lee, Csaba Balázsi, Katalin Balázsi, Dong hyun Seo, Hansung Kim, Chang Hyen Kim, Seong Gon Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The aims of this study were to determine the physical properties of hydroxyapatite from seashells (sHA) and from eggshells (eHA), to analyze elements within sHA and eHA, and to compare the bone regeneration ability between sHA and eHA in a rat parietal bone defect model. The sHA and eHA particles had a similar morphology in scanning electron microscope images. From the Fourier-transform infrared absorbance spectra and X-ray diffraction results, both types of hydroxyapatite (HA) had the characteristics of pure HA. Inductively coupled plasma atomic emission spectroscopy results suggested that the sHA had higher levels of sodium and strontium than the eHA, whereas the eHA had higher levels of magnesium than the sHA. In μ-CT results, the mean bone mineral density of the sHA was significantly higher than the control at 4 weeks after the operation (p = 0.012). The mean bone volume of the eHA was significantly higher than the control at 8 weeks after the operation (p = 0.012). In the histological images at 4 weeks after the operation, foreign body multinucleated giant cells were observed around the agglomerated sHA particles, while there were fewer inflammatory reactions around the agglomerated eHA particles. The eHA group showed better results in bone formation than did the sHA group in this study.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalTissue Engineering and Regenerative Medicine
Volume11
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Animal Shells
Egg Shell
Durapatite
Hydroxyapatite
Grafts
Bone
Transplants
Bone and Bones
Foreign Body Giant Cells
Parietal Bone
Atomic Spectrophotometry
Strontium
Bone Regeneration
Fourier Analysis
Giant Cells
Osteogenesis
X-Ray Diffraction
Bone Density
Magnesium
Atomic emission spectroscopy

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Lee, Sang Woon ; Balázsi, Csaba ; Balázsi, Katalin ; Seo, Dong hyun ; Kim, Hansung ; Kim, Chang Hyen ; Kim, Seong Gon. / Comparative Study of hydroxyapatite prepared from seashells and eggshells as a bone graft material. In: Tissue Engineering and Regenerative Medicine. 2014 ; Vol. 11, No. 2. pp. 113-120.
@article{e5da014401a44f2089d443ea284527dc,
title = "Comparative Study of hydroxyapatite prepared from seashells and eggshells as a bone graft material",
abstract = "The aims of this study were to determine the physical properties of hydroxyapatite from seashells (sHA) and from eggshells (eHA), to analyze elements within sHA and eHA, and to compare the bone regeneration ability between sHA and eHA in a rat parietal bone defect model. The sHA and eHA particles had a similar morphology in scanning electron microscope images. From the Fourier-transform infrared absorbance spectra and X-ray diffraction results, both types of hydroxyapatite (HA) had the characteristics of pure HA. Inductively coupled plasma atomic emission spectroscopy results suggested that the sHA had higher levels of sodium and strontium than the eHA, whereas the eHA had higher levels of magnesium than the sHA. In μ-CT results, the mean bone mineral density of the sHA was significantly higher than the control at 4 weeks after the operation (p = 0.012). The mean bone volume of the eHA was significantly higher than the control at 8 weeks after the operation (p = 0.012). In the histological images at 4 weeks after the operation, foreign body multinucleated giant cells were observed around the agglomerated sHA particles, while there were fewer inflammatory reactions around the agglomerated eHA particles. The eHA group showed better results in bone formation than did the sHA group in this study.",
author = "Lee, {Sang Woon} and Csaba Bal{\'a}zsi and Katalin Bal{\'a}zsi and Seo, {Dong hyun} and Hansung Kim and Kim, {Chang Hyen} and Kim, {Seong Gon}",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/s13770-014-0056-1",
language = "English",
volume = "11",
pages = "113--120",
journal = "Tissue Engineering and Regenerative Medicine",
issn = "1738-2696",
publisher = "Springer Science + Business Media",
number = "2",

}

Comparative Study of hydroxyapatite prepared from seashells and eggshells as a bone graft material. / Lee, Sang Woon; Balázsi, Csaba; Balázsi, Katalin; Seo, Dong hyun; Kim, Hansung; Kim, Chang Hyen; Kim, Seong Gon.

In: Tissue Engineering and Regenerative Medicine, Vol. 11, No. 2, 01.01.2014, p. 113-120.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparative Study of hydroxyapatite prepared from seashells and eggshells as a bone graft material

AU - Lee, Sang Woon

AU - Balázsi, Csaba

AU - Balázsi, Katalin

AU - Seo, Dong hyun

AU - Kim, Hansung

AU - Kim, Chang Hyen

AU - Kim, Seong Gon

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The aims of this study were to determine the physical properties of hydroxyapatite from seashells (sHA) and from eggshells (eHA), to analyze elements within sHA and eHA, and to compare the bone regeneration ability between sHA and eHA in a rat parietal bone defect model. The sHA and eHA particles had a similar morphology in scanning electron microscope images. From the Fourier-transform infrared absorbance spectra and X-ray diffraction results, both types of hydroxyapatite (HA) had the characteristics of pure HA. Inductively coupled plasma atomic emission spectroscopy results suggested that the sHA had higher levels of sodium and strontium than the eHA, whereas the eHA had higher levels of magnesium than the sHA. In μ-CT results, the mean bone mineral density of the sHA was significantly higher than the control at 4 weeks after the operation (p = 0.012). The mean bone volume of the eHA was significantly higher than the control at 8 weeks after the operation (p = 0.012). In the histological images at 4 weeks after the operation, foreign body multinucleated giant cells were observed around the agglomerated sHA particles, while there were fewer inflammatory reactions around the agglomerated eHA particles. The eHA group showed better results in bone formation than did the sHA group in this study.

AB - The aims of this study were to determine the physical properties of hydroxyapatite from seashells (sHA) and from eggshells (eHA), to analyze elements within sHA and eHA, and to compare the bone regeneration ability between sHA and eHA in a rat parietal bone defect model. The sHA and eHA particles had a similar morphology in scanning electron microscope images. From the Fourier-transform infrared absorbance spectra and X-ray diffraction results, both types of hydroxyapatite (HA) had the characteristics of pure HA. Inductively coupled plasma atomic emission spectroscopy results suggested that the sHA had higher levels of sodium and strontium than the eHA, whereas the eHA had higher levels of magnesium than the sHA. In μ-CT results, the mean bone mineral density of the sHA was significantly higher than the control at 4 weeks after the operation (p = 0.012). The mean bone volume of the eHA was significantly higher than the control at 8 weeks after the operation (p = 0.012). In the histological images at 4 weeks after the operation, foreign body multinucleated giant cells were observed around the agglomerated sHA particles, while there were fewer inflammatory reactions around the agglomerated eHA particles. The eHA group showed better results in bone formation than did the sHA group in this study.

UR - http://www.scopus.com/inward/record.url?scp=84898483268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898483268&partnerID=8YFLogxK

U2 - 10.1007/s13770-014-0056-1

DO - 10.1007/s13770-014-0056-1

M3 - Article

VL - 11

SP - 113

EP - 120

JO - Tissue Engineering and Regenerative Medicine

JF - Tissue Engineering and Regenerative Medicine

SN - 1738-2696

IS - 2

ER -