Comparative study on metabolite level in tissue-specific human mesenchymal stem cells by an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry

Seul Ji Lee, Tac Ghee Yi, Soo Hyun Ahn, Dong Kyu Lim, Si na Kim, Hyun Joo Lee, Yun Kyoung Cho, JaeYoul Lim, Jong Hyuk Sung, Jeong Ho Yun, Johan Lim, Sun U. Song, Sung Won Kwon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characteristics, little is known about the biological differences between MSC populations derived from different tissues. In this study, we used MS to compare the endogenous metabolite level in the human MSCs originating from the bone marrow, adipose tissue, periodontal ligaments, and salivary glands. Using an optimized metabolomics technique, we verified that human MSCs exhibit differences in the endogenous metabolite level depending on their source material, while the multivariate analysis showed that 5 lysophosphatidylcholines and 3 lysophosphatidylethanolamines can serve as markers for the discrimination between MSC sources and may be related to differences in their differentiation capacity. These results may significantly contribute to further mechanistic studies on the MSCs and provide novel insights into the properties and optimal usage of MSCs from different tissues.

Original languageEnglish
Pages (from-to)112-122
Number of pages11
JournalAnalytica Chimica Acta
Volume1024
DOIs
Publication statusPublished - 2018 Sep 18

Fingerprint

Liquid chromatography
Metabolites
Stem cells
Mesenchymal Stromal Cells
Liquid Chromatography
Mass spectrometry
liquid chromatography
Mass Spectrometry
metabolite
comparative study
mass spectrometry
stem
Tissue
bone
Bone
Bone Marrow
Periodontal Ligament
Lysophosphatidylcholines
Metabolomics
multivariate analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Lee, Seul Ji ; Yi, Tac Ghee ; Ahn, Soo Hyun ; Lim, Dong Kyu ; Kim, Si na ; Lee, Hyun Joo ; Cho, Yun Kyoung ; Lim, JaeYoul ; Sung, Jong Hyuk ; Yun, Jeong Ho ; Lim, Johan ; Song, Sun U. ; Kwon, Sung Won. / Comparative study on metabolite level in tissue-specific human mesenchymal stem cells by an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry. In: Analytica Chimica Acta. 2018 ; Vol. 1024. pp. 112-122.
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Comparative study on metabolite level in tissue-specific human mesenchymal stem cells by an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry. / Lee, Seul Ji; Yi, Tac Ghee; Ahn, Soo Hyun; Lim, Dong Kyu; Kim, Si na; Lee, Hyun Joo; Cho, Yun Kyoung; Lim, JaeYoul; Sung, Jong Hyuk; Yun, Jeong Ho; Lim, Johan; Song, Sun U.; Kwon, Sung Won.

In: Analytica Chimica Acta, Vol. 1024, 18.09.2018, p. 112-122.

Research output: Contribution to journalArticle

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AU - Lee, Seul Ji

AU - Yi, Tac Ghee

AU - Ahn, Soo Hyun

AU - Lim, Dong Kyu

AU - Kim, Si na

AU - Lee, Hyun Joo

AU - Cho, Yun Kyoung

AU - Lim, JaeYoul

AU - Sung, Jong Hyuk

AU - Yun, Jeong Ho

AU - Lim, Johan

AU - Song, Sun U.

AU - Kwon, Sung Won

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