Comparative study on metabolite level in tissue-specific human mesenchymal stem cells by an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry

Seul Ji Lee, Tac Ghee Yi, Soo Hyun Ahn, Dong Kyu Lim, Si na Kim, Hyun Joo Lee, Yun Kyoung Cho, Jae Yol Lim, Jong Hyuk Sung, Jeong Ho Yun, Johan Lim, Sun U. Song, Sung Won Kwon

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characteristics, little is known about the biological differences between MSC populations derived from different tissues. In this study, we used MS to compare the endogenous metabolite level in the human MSCs originating from the bone marrow, adipose tissue, periodontal ligaments, and salivary glands. Using an optimized metabolomics technique, we verified that human MSCs exhibit differences in the endogenous metabolite level depending on their source material, while the multivariate analysis showed that 5 lysophosphatidylcholines and 3 lysophosphatidylethanolamines can serve as markers for the discrimination between MSC sources and may be related to differences in their differentiation capacity. These results may significantly contribute to further mechanistic studies on the MSCs and provide novel insights into the properties and optimal usage of MSCs from different tissues.

Original languageEnglish
Pages (from-to)112-122
Number of pages11
JournalAnalytica Chimica Acta
Volume1024
DOIs
Publication statusPublished - 2018 Sep 18

Bibliographical note

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [grant number NRF-2017R1A6A3A11028026]; the Bio-Synergy Research Project of the Ministry of Science, ICT and Future Planning through the National Research Foundation [grant number NRF-2012M3A9C4048796]; and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health and Welfare, Republic of Korea [grant number HI16C1894].

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [grant number NRF-2017R1A6A3A11028026 ]; the Bio-Synergy Research Project of the Ministry of Science, ICT and Future Planning through the National Research Foundation [grant number NRF-2012M3A9C4048796 ]; and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health and Welfare, Republic of Korea [grant number HI16C1894 ].

Publisher Copyright:
© 2018 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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