Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography

Philjun Jeon, Jongwu Kim, Heejung Lee, Hyuk Sang Kwon, Dug Young Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-µm diameter.

Original languageEnglish
Pages (from-to)9231-9241
Number of pages11
JournalOptics Express
Volume29
Issue number6
DOIs
Publication statusPublished - 2021 Mar 15

Bibliographical note

Funding Information:
National Research Foundation of Korea (2017R1A2B4003950); Korea Institute for Advancement of Technology (P0011925).

Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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