Comparative transcriptome analysis of the CO2 sensing pathway via differential expression of carbonic anhydrase in Cryptococcus neoformans

Min Su Kim, Young Joon Ko, Shinae Maeng, Anna Floyd, Joseph Heitman, Yong-Sun Bahn

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Carbon dioxide (CO2) sensing and metabolism via carbonic anhydrases (CAs) play pivotal roles in survival and proliferation of pathogenic fungi infecting human hosts from natural environments due to the drastic difference in CO2 levels. In Cryptococcus neoformans, which causes fatal fungal meningoencephalitis, the Can2 CA plays essential roles during both cellular growth in air and sexual differentiation of the pathogen. However the signaling networks downstream of Can2 are largely unknown. To address this question, the present study employed comparative transcriptome DNA microarray analysis of a C. neoformans strain in which CAN2 expression is artificially controlled by the CTR4 (copper transporter) promoter. The PCTR4::CAN2 strain showed growth defects in a CO2-dependent manner when CAN2 was repressed but resumed normal growth when CAN2 was overexpressed. The Can2-dependent genes identified by the transcriptome analysis include FAS1 (fatty acid synthase 1) and GPB1 (G-protein β subunit), supporting the roles of Can2 in fatty acid biosynthesis and sexual differentiation. Cas3, a capsular structure designer protein, was also discovered to be Can2-dependent and yet was not involved in CO2-mediated capsule induction. Most notably, a majority of Can2-dependent genes were environmental stress-regulated (ESR) genes. Supporting this, the CAN2 overexpression strain was hypersensitive to oxidative and genotoxic stress as well as antifungal drugs, such as polyene and azole drugs, potentially due to defective membrane integrity. Finally, an oxidative stress-responsive Atf1 transcription factor was also found to be Can2-dependent. Atf1 not only plays an important role in diverse stress responses, including thermotolerance and antifungal drug resistance, but also represses melanin and capsule production in C. neoformans. In conclusion, this study provides insights into the comprehensive signaling networks orchestrated by CA/CO2-sensing pathways in pathogenic fungi.

Original languageEnglish
Pages (from-to)1207-1219
Number of pages13
JournalGenetics
Volume185
Issue number4
DOIs
Publication statusPublished - 2010 Aug 1

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Cryptococcus neoformans
Carbonic Anhydrases
Gene Expression Profiling
Sex Differentiation
Capsules
Fungal Drug Resistance
Oxidative Stress
Fungi
Growth
Genes
Polyenes
Fatty Acid Synthases
Azoles
Meningoencephalitis
Melanins
Protein Subunits
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Transcriptome
GTP-Binding Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Kim, Min Su ; Ko, Young Joon ; Maeng, Shinae ; Floyd, Anna ; Heitman, Joseph ; Bahn, Yong-Sun. / Comparative transcriptome analysis of the CO2 sensing pathway via differential expression of carbonic anhydrase in Cryptococcus neoformans. In: Genetics. 2010 ; Vol. 185, No. 4. pp. 1207-1219.
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Comparative transcriptome analysis of the CO2 sensing pathway via differential expression of carbonic anhydrase in Cryptococcus neoformans. / Kim, Min Su; Ko, Young Joon; Maeng, Shinae; Floyd, Anna; Heitman, Joseph; Bahn, Yong-Sun.

In: Genetics, Vol. 185, No. 4, 01.08.2010, p. 1207-1219.

Research output: Contribution to journalArticle

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