Escherichia coli, Klebsiella pneumoniae Korean Source Proteus mirabilis Korean Source AmpC β-lactamase Korean Source 3 Korean Source

Translated title of the contribution: Comparison of 3 phenotypic-detection methods for identifying plasmid-mediated AmpC β-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis strains

Wookeun Lee, Bochan Jung, Seong Geun Hong, Wonkeun Song, Seokhoon Jeong, Kyungwon Lee, Hyo Sun Kwak

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. Methods: Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. Results: PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. Conclusions: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.

Original languageKorean
Pages (from-to)448-454
Number of pages7
JournalKorean Journal of Laboratory Medicine
Volume29
Issue number5
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Proteus mirabilis
Klebsiella pneumoniae
Escherichia coli
Plasmids
Cephamycins
Edetic Acid
Multiplex Polymerase Chain Reaction
Genes
Cefotetan
Clinical laboratories
Cefoxitin
Salmonella
Klebsiella
Korea
Infection Control
Phenotype
Sensitivity and Specificity
3-aminobenzeneboronic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry, medical
  • Clinical Biochemistry

Cite this

@article{f361e9ee527745ddb37ca028cff2b68e,
title = "Escherichia coli, Klebsiella pneumoniae Korean Source Proteus mirabilis Korean Source AmpC β-lactamase Korean Source 3 Korean Source",
abstract = "Background: Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. Methods: Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. Results: PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4{\%}), specificity (92.2{\%}), and efficiency (96.3{\%}) than the cephamycin-Hodge (76.2{\%}, 96.1{\%}, and 88.6{\%}, respectively) and the TE-disk (80.3{\%}, 91.6{\%}, and 87.9{\%}, respectively) tests. Conclusions: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.",
author = "Wookeun Lee and Bochan Jung and Hong, {Seong Geun} and Wonkeun Song and Seokhoon Jeong and Kyungwon Lee and Kwak, {Hyo Sun}",
year = "2009",
month = "12",
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language = "Korean",
volume = "29",
pages = "448--454",
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publisher = "Seoul National University",
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Escherichia coli, Klebsiella pneumoniae Korean Source Proteus mirabilis Korean Source AmpC β-lactamase Korean Source 3 Korean Source. / Lee, Wookeun; Jung, Bochan; Hong, Seong Geun; Song, Wonkeun; Jeong, Seokhoon; Lee, Kyungwon; Kwak, Hyo Sun.

In: Korean Journal of Laboratory Medicine, Vol. 29, No. 5, 01.12.2009, p. 448-454.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Escherichia coli, Klebsiella pneumoniae Korean Source Proteus mirabilis Korean Source AmpC β-lactamase Korean Source 3 Korean Source

AU - Lee, Wookeun

AU - Jung, Bochan

AU - Hong, Seong Geun

AU - Song, Wonkeun

AU - Jeong, Seokhoon

AU - Lee, Kyungwon

AU - Kwak, Hyo Sun

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Background: Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. Methods: Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. Results: PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. Conclusions: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.

AB - Background: Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. Methods: Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. Results: PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. Conclusions: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.

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