Comparison of a conventional antimicrobial susceptibility assay to an oligonucleotide chip system for detection of drug resistance in Mycobacterium tuberculosis isolates

Heekyung Park, Eun Ju Song, Eun Sil Song, Eun Yup Lee, Cheol Min Kim, Seok Hoon Jeong, Jeong Hwan Shin, Joseph Jeong, Sunjoo Kim, Young Kil Park, Gill Han Bai, Chulhun L. Chang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

An oligonucleotide chip (Combichip Mycobacteria chip) detecting specific mutations in the rpoB, katG, and inhA genes of Mycobacterium tuberculosis was compared with conventional antimicrobial susceptibility results. The probes detecting drug resistance were as follows: 7 wild-type and 13 mutant probes for rifampin and 2 wild-type and 3 mutant probes for isoniazid. Target DNA of M. tuberculosis was amplified by PCR, followed by hybridization and scanning. Direct sequencing was performed to verify the results of the oligonucleotide chip. One-hundred seven of 115 rifampin-resistant strains (93%) had mutations in the rpoB gene. Eighty-five of 119 isoniazid-resistant strains (71%) had mutations in the katG gene or inhA gene. The diagnostic oligonucleotide chip with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of resistance against rifampin and isoniazid in M. tuberculosis isolates.

Original languageEnglish
Pages (from-to)1619-1624
Number of pages6
JournalJournal of Clinical Microbiology
Volume44
Issue number5
DOIs
Publication statusPublished - 2006 May

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Comparison of a conventional antimicrobial susceptibility assay to an oligonucleotide chip system for detection of drug resistance in Mycobacterium tuberculosis isolates'. Together they form a unique fingerprint.

  • Cite this