Comparison of allergenic properties among commercially available house dust mite allergen extracts in korea

Ji Tae Kim, Hyunho Kim, Sung Hyun Kim, Dong Jun Kim, Yeji Shin, Jung Dong Kim, Hangyeol Song, Seok Woo Jang, Deug Chan Lee, Kyung Hee Park, Jae Hyun Lee, Kyoung Yong Jeong, Jung Won Park

Research output: Contribution to journalArticlepeer-review

Abstract

Precise allergy diagnosis and effective allergen specific immunotherapy are largely dependent on the quality of allergen extract. A new extract of Dermatophagoides farinae was commercially developed by Prolagen. The allergenic properties of the new extract were compared with those of other commercial products. The allergenic properties of the new extract were compared according to protein concentration, protein profiles, major allergen (Der f 1) contents, and allergenic potency to those for three commercially available extracts imported in Korea (Jubilant HollisterStier Allergy, Lofarma S.p.A., and Stallergenes Greer). Protein concentrations varied up to 2.62-fold (0.404 to 1.057 mg/mL), and Der f 1 contents varied up to 11.3-fold (3.597 to 40.688 μg/mL). Protein profiles of the extracts showed no major discrepancies, although there were some differences in SDS-PAGE band intensi-ties, reflecting protein concentrations. Allergen potency ranged from 37038 to 60491 PAU/mL. The Prolagen product was highest in terms of protein concentration and allergen potency. The Lofarma product displayed Der f 1 content similar to that in Prolagen (19.4 μg/mg vs. 19.3 μg/mg). Endotoxin levels varied 8.9-fold (1020 to 8985 EU/mL). The newly developed house dust mite extract showed equal or better allergenic properties than available commercial extracts. This new product may be useful for better diag-nostics and allergen-specific immunotherapeutics.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalYonsei medical journal
Volume62
Issue number1
DOIs
Publication statusPublished - 2021 Jan

Bibliographical note

Funding Information:
This work was supported by the Technology Development Program (S2765576) funded by the Ministry of SMEs and Startups (MSS, Korea). This work was also supported by the Medical Device Technology Development Program (20006057, Highly sensitive three dimensional fluorescent chip for multiple allergy diagnosis) funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea).

Publisher Copyright:
© Yonsei University College of Medicine 2021.

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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