Comparison of biological phenotypes according to midkine expression in gastric cancer cells and their autocrine activities could be modulated by pentosan polysulfate

SunYoung Rha, Sung Hoon Noh, Hyun Joo Kwak, Anton Wellstein, Joo Hang Kim, Jae Kyung Roh, Jin Sik Min, Byung Soo Kim, Hyuncheol Chung

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied biological phenotypes of gastric cancer cell lines based on a novel heparin-binding growth/differentiation factor (midkine (MK)) expression. MK expression was found in 67% (6/9) of the gastric cancer cell lines and 56% (14/25) of the primary cancer tissues. Gastric cancer cell lines with MK expression showed higher colony forming activity in soft agar assay and endothelial cell growth stimulatory effect in cross-feeding assay than cells which did not express MK. However, urokinase-type plasminogen activator (uPA) expression and tumor invasiveness did not correlate with MK expression. Growth of MK expressing cells was inhibited by a heparin-binding blocking agent, pentosan polysulfate (PPS). In cancer tissues, MK expression correlated with tumor size, suggesting in vivo autocrine and paracrine activity. This proliferation promoting activity of MK can be targeted by an anti-heparin binding agent as a biotherapy model in gastric cancer.

Original languageEnglish
Pages (from-to)37-46
Number of pages10
JournalCancer Letters
Volume118
Issue number1
DOIs
Publication statusPublished - 1997 Sep 16

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Pentosan Sulfuric Polyester
Stomach Neoplasms
Phenotype
Heparin
Cell Line
Neoplasms
Growth Differentiation Factors
Biological Therapy
midkine
Urokinase-Type Plasminogen Activator
Growth
Agar
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Rha, SunYoung ; Noh, Sung Hoon ; Kwak, Hyun Joo ; Wellstein, Anton ; Kim, Joo Hang ; Roh, Jae Kyung ; Min, Jin Sik ; Kim, Byung Soo ; Chung, Hyuncheol. / Comparison of biological phenotypes according to midkine expression in gastric cancer cells and their autocrine activities could be modulated by pentosan polysulfate. In: Cancer Letters. 1997 ; Vol. 118, No. 1. pp. 37-46.
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Comparison of biological phenotypes according to midkine expression in gastric cancer cells and their autocrine activities could be modulated by pentosan polysulfate. / Rha, SunYoung; Noh, Sung Hoon; Kwak, Hyun Joo; Wellstein, Anton; Kim, Joo Hang; Roh, Jae Kyung; Min, Jin Sik; Kim, Byung Soo; Chung, Hyuncheol.

In: Cancer Letters, Vol. 118, No. 1, 16.09.1997, p. 37-46.

Research output: Contribution to journalArticle

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AU - Kim, Joo Hang

AU - Roh, Jae Kyung

AU - Min, Jin Sik

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AU - Chung, Hyuncheol

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