Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea

Young Kil Park, Sonya Shin, Sungweon Ryu, Nae Cho Sang, Won Jung Koh, Jung Kwon O, Soo Shim Young, Jin Lew Woo, Han Bai Gill

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Abstract

The frequency of resistance genotypes among Beijing and non-Beijing strains was compared using a reverse blot hybridization assay to detect mutations within genes associated with rifampicin (rpoB) and isoniazid (katG, inhA, and ahpC) resistance. Of the 743 Mycobacterium tuberculosis isolates, 569 (77%) belonged to Beijing family. The proportion of Beijing strains was significantly higher among MDR-TB isolates than among drug-susceptible strains (82% vs. 72%, p < 0.01). Genotype analysis of the rpoB gene revealed significantly lower rates of the Ser531Leu mutation rate among Beijing vs. non-Beijing MDR-TB strains (41% vs. 66%, p < 0.005). While the mutation for Ser315Thr in the katG gene was more common among Beijing vs. non-Beijing family strains (65% vs. 50%, p < 0.01), the mutation rate of promoter region of the inhA gene was lower among Beijing strains compared with non-Beijing strains (14% vs. 25%, p < 0.05). Reverse hybridization successfully detected over 80% of isoniazid-resistant strains and over 92% of rifampicin-resistant strains among Korean isolates. Significant differences in mutation rates in the rpoB, katG, and inhA genes between Beijing strains and non-Beijing strains could explain discrepancies in mutation rates of genotypes in different countries. Reverse hybridization was useful for rapid detection of isoniazid and rifampicin resistant strains.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalJournal of Microbiological Methods
Volume63
Issue number2
DOIs
Publication statusPublished - 2005 Nov 1

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Korea
Mycobacterium tuberculosis
Drug Resistance
Genotype
Mutation Rate
Isoniazid
Rifampin
Genes
Mutation
Beijing
Genetic Promoter Regions
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Park, Young Kil ; Shin, Sonya ; Ryu, Sungweon ; Sang, Nae Cho ; Koh, Won Jung ; O, Jung Kwon ; Young, Soo Shim ; Woo, Jin Lew ; Gill, Han Bai. / Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea. In: Journal of Microbiological Methods. 2005 ; Vol. 63, No. 2. pp. 165-172.
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Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea. / Park, Young Kil; Shin, Sonya; Ryu, Sungweon; Sang, Nae Cho; Koh, Won Jung; O, Jung Kwon; Young, Soo Shim; Woo, Jin Lew; Gill, Han Bai.

In: Journal of Microbiological Methods, Vol. 63, No. 2, 01.11.2005, p. 165-172.

Research output: Contribution to journalArticle

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AU - Park, Young Kil

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