Comparison of E,E-farnesol secretion and the clinical characteristics of candida albicans bloodstream isolates from different multilocus sequence typing clades

Sook In Jung, Jong Hee Shin, Soo Hyun Kim, Jin Kim, Joo Hee Kim, Min Ji Choi, Eun Kyung Chung, Kyungwon Lee, Sun Hoe Koo, Hyun Ha Chang, Marie Elisabeth Bougnoux, Christophe D'Enfert

Research output: Contribution to journalArticle

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Abstract

Using multilocus sequence typing (MLST), Candida albicans can be subdivided into 18 different clades. Farnesol, a quorum-sensing molecule secreted by C. albicans, is thought to play an important role in the development of C. albicans biofilms and is also a virulence factor. This study evaluated whether C. albicans bloodstream infection (BSI) strains belonging to different MLST clades secrete different levels of E,E-farnesol (FOH) and whether they have different clinical characteristics. In total, 149 C. albicans BSI isolates from ten Korean hospitals belonging to clades 18 (n = 28), 4 (n = 23), 1 (n = 22), 12 (n = 17), and other clades (n = 59) were assessed. For each isolate, the FOH level in 24-hour biofilms was determined in filtered (0.45 μm) culture supernatant using high-performance liquid chromatography. Marked differences in FOH secretion from biofilms (0.10â€"6.99 μM) were observed among the 149 BSI isolates. Clade 18 isolates secreted significantly more FOH than did non-clade 18 isolates (mean ± SEM; 2.66 ± 0.22 vs. 1.69 ± 0.10 μM; P < 0.001). Patients with isolates belonging to clade 18 had a lower mean severity of illness than other patients, as measured using the íacute physiology and chronic health evaluation" (APACHE) III score (14.4 ± 1.1 vs. 18.0 ± 0.7; P < 0.05). This study provides evidence that C. albicans BSI isolates belonging to the most prevalent MLST clade (clade 18) in Korea are characterized by increased levels of FOH secretion and less severe illness.

Original languageEnglish
Article numbere0148400
JournalPloS one
Volume11
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Farnesol
farnesol
Multilocus Sequence Typing
Candida
Biofilms
Candida albicans
blood flow
secretion
biofilm
Physiology
High performance liquid chromatography
Virulence Factors
Infection
infection
Health
Quorum Sensing
Scanning electron microscopy
Molecules
APACHE
quorum sensing

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jung, Sook In ; Shin, Jong Hee ; Kim, Soo Hyun ; Kim, Jin ; Kim, Joo Hee ; Choi, Min Ji ; Chung, Eun Kyung ; Lee, Kyungwon ; Koo, Sun Hoe ; Chang, Hyun Ha ; Bougnoux, Marie Elisabeth ; D'Enfert, Christophe. / Comparison of E,E-farnesol secretion and the clinical characteristics of candida albicans bloodstream isolates from different multilocus sequence typing clades. In: PloS one. 2016 ; Vol. 11, No. 2.
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abstract = "Using multilocus sequence typing (MLST), Candida albicans can be subdivided into 18 different clades. Farnesol, a quorum-sensing molecule secreted by C. albicans, is thought to play an important role in the development of C. albicans biofilms and is also a virulence factor. This study evaluated whether C. albicans bloodstream infection (BSI) strains belonging to different MLST clades secrete different levels of E,E-farnesol (FOH) and whether they have different clinical characteristics. In total, 149 C. albicans BSI isolates from ten Korean hospitals belonging to clades 18 (n = 28), 4 (n = 23), 1 (n = 22), 12 (n = 17), and other clades (n = 59) were assessed. For each isolate, the FOH level in 24-hour biofilms was determined in filtered (0.45 μm) culture supernatant using high-performance liquid chromatography. Marked differences in FOH secretion from biofilms (0.10{\^a}€{"}6.99 μM) were observed among the 149 BSI isolates. Clade 18 isolates secreted significantly more FOH than did non-clade 18 isolates (mean ± SEM; 2.66 ± 0.22 vs. 1.69 ± 0.10 μM; P < 0.001). Patients with isolates belonging to clade 18 had a lower mean severity of illness than other patients, as measured using the {\'i}acute physiology and chronic health evaluation{"} (APACHE) III score (14.4 ± 1.1 vs. 18.0 ± 0.7; P < 0.05). This study provides evidence that C. albicans BSI isolates belonging to the most prevalent MLST clade (clade 18) in Korea are characterized by increased levels of FOH secretion and less severe illness.",
author = "Jung, {Sook In} and Shin, {Jong Hee} and Kim, {Soo Hyun} and Jin Kim and Kim, {Joo Hee} and Choi, {Min Ji} and Chung, {Eun Kyung} and Kyungwon Lee and Koo, {Sun Hoe} and Chang, {Hyun Ha} and Bougnoux, {Marie Elisabeth} and Christophe D'Enfert",
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Jung, SI, Shin, JH, Kim, SH, Kim, J, Kim, JH, Choi, MJ, Chung, EK, Lee, K, Koo, SH, Chang, HH, Bougnoux, ME & D'Enfert, C 2016, 'Comparison of E,E-farnesol secretion and the clinical characteristics of candida albicans bloodstream isolates from different multilocus sequence typing clades', PloS one, vol. 11, no. 2, e0148400. https://doi.org/10.1371/journal.pone.0148400

Comparison of E,E-farnesol secretion and the clinical characteristics of candida albicans bloodstream isolates from different multilocus sequence typing clades. / Jung, Sook In; Shin, Jong Hee; Kim, Soo Hyun; Kim, Jin; Kim, Joo Hee; Choi, Min Ji; Chung, Eun Kyung; Lee, Kyungwon; Koo, Sun Hoe; Chang, Hyun Ha; Bougnoux, Marie Elisabeth; D'Enfert, Christophe.

In: PloS one, Vol. 11, No. 2, e0148400, 01.02.2016.

Research output: Contribution to journalArticle

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T1 - Comparison of E,E-farnesol secretion and the clinical characteristics of candida albicans bloodstream isolates from different multilocus sequence typing clades

AU - Jung, Sook In

AU - Shin, Jong Hee

AU - Kim, Soo Hyun

AU - Kim, Jin

AU - Kim, Joo Hee

AU - Choi, Min Ji

AU - Chung, Eun Kyung

AU - Lee, Kyungwon

AU - Koo, Sun Hoe

AU - Chang, Hyun Ha

AU - Bougnoux, Marie Elisabeth

AU - D'Enfert, Christophe

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Using multilocus sequence typing (MLST), Candida albicans can be subdivided into 18 different clades. Farnesol, a quorum-sensing molecule secreted by C. albicans, is thought to play an important role in the development of C. albicans biofilms and is also a virulence factor. This study evaluated whether C. albicans bloodstream infection (BSI) strains belonging to different MLST clades secrete different levels of E,E-farnesol (FOH) and whether they have different clinical characteristics. In total, 149 C. albicans BSI isolates from ten Korean hospitals belonging to clades 18 (n = 28), 4 (n = 23), 1 (n = 22), 12 (n = 17), and other clades (n = 59) were assessed. For each isolate, the FOH level in 24-hour biofilms was determined in filtered (0.45 μm) culture supernatant using high-performance liquid chromatography. Marked differences in FOH secretion from biofilms (0.10â€"6.99 μM) were observed among the 149 BSI isolates. Clade 18 isolates secreted significantly more FOH than did non-clade 18 isolates (mean ± SEM; 2.66 ± 0.22 vs. 1.69 ± 0.10 μM; P < 0.001). Patients with isolates belonging to clade 18 had a lower mean severity of illness than other patients, as measured using the íacute physiology and chronic health evaluation" (APACHE) III score (14.4 ± 1.1 vs. 18.0 ± 0.7; P < 0.05). This study provides evidence that C. albicans BSI isolates belonging to the most prevalent MLST clade (clade 18) in Korea are characterized by increased levels of FOH secretion and less severe illness.

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