Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae

Sung Geun Woo, Keunje Yoo, Juyoun Lee, Sara Bang, Myungjin Lee, Kwangchul On, Joonhong Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalTalanta
Volume97
DOIs
Publication statusPublished - 2012 Aug 15

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Microalgae
Waste Water
Gas chromatography
Gas Chromatography
Wastewater
Fatty Acids
Mass Spectrometry
Scenedesmus
Chlorella vulgaris
Spectroscopy
Unsaturated Fatty Acids
Culture Media
Limit of Detection
Nitrogen
Detectors
Chemical analysis
Research

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Woo, Sung Geun ; Yoo, Keunje ; Lee, Juyoun ; Bang, Sara ; Lee, Myungjin ; On, Kwangchul ; Park, Joonhong. / Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae. In: Talanta. 2012 ; Vol. 97. pp. 103-110.
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Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae. / Woo, Sung Geun; Yoo, Keunje; Lee, Juyoun; Bang, Sara; Lee, Myungjin; On, Kwangchul; Park, Joonhong.

In: Talanta, Vol. 97, 15.08.2012, p. 103-110.

Research output: Contribution to journalArticle

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