Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae

Wonmok Lee, Hae Sun Chung, Yangsoon Lee, DongEun Yong, Seokhoon Jeong, Kyungwon Lee, Yunsop Chong

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.

Original languageEnglish
Pages (from-to)227-230
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume77
Issue number3
DOIs
Publication statusPublished - 2013 Nov 1

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Inosine Monophosphate
Acinetobacter
Klebsiella pneumoniae
Pseudomonas aeruginosa
Mass Spectrometry
Lasers
Carbapenems
Gram-Negative Bacteria
Biological Assay
Asp(5)-oxytocin
carbapenemase
ertapenem

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae",
abstract = "Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.",
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AU - Chung, Hae Sun

AU - Lee, Yangsoon

AU - Yong, DongEun

AU - Jeong, Seokhoon

AU - Lee, Kyungwon

AU - Chong, Yunsop

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AB - Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.

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