Comparison of QFT-Plus and QFT-GIT tests for diagnosis of M. tuberculosis infection in immunocompetent Korean subjects

Ji Young Hong, So Yeong Park, Ahreum Kim, Sang Nae Cho, Yun Gyoung Hur

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background: QFT-Plus is a recently developed next-generation QuantiFERON-TB Gold In-Tube (QFT-GIT) test. Unlike the QFT-GIT test, it includes a TB2 antigen tube with peptides that may stimulate CD8+ T cells. This study evaluated the diagnostic performance of QFT-Plus and compared it with that of QFT-GIT. Methods: QFT-Plus and QFT-GIT tests were performed in 33 patients with active tuberculosis (TB) and 57 healthy controls including subjects with latent TB infection (LTBI). Positivity and negativity of IFN-γ responses were compared between tests, and total concordance of the outcome was analyzed. Results: Positive and negative outcomes of QFT-Plus and QFT-GIT tests showed substantial agreement (91.1%, kappa=0.8). The sensitivity and the specificity of QFT-Plus (93.9% sensitivity, 92.6% specificity) were similar with those of QFT-GIT (93.9% sensitivity, 100% specificity). Of eight discordant results, five (5.56%) and three (3.3%) were positive in QFT-GIT alone and QFT-plus alone, respectively. Reactivity in the TB2 tube contributes to the difference between QFT-GIT and QFT-Plus. Median IFN-γ production in TB2 (10.0 IU/mL in TB, 3.850 IU/mL in LTBI, P=0.001) is significantly higher in the TB group than the LTBI group. The QFT-Plus did not clearly discriminate between active TB and latent TB, although it showed significantly lower IFN-γ concentrations compared with the QFT-GIT in individuals with LTBI (3.850 vs. 7.205 IU/mL). Conclusions: Similar accuracy of detecting Mycobacterium tuberculosis infection was observed for QFT-Plus and QFT-GIT tests in immunocompetent patients and healthy controls, including those with LTBI. Improved efficacy for identifying M. tuberculosis infection was not found with the QFT-Plus, but further studies in a larger population may confirm the clinical significance of positive response in the TB2 tube of QFT-Plus.

Original languageEnglish
Pages (from-to)5210-5217
Number of pages8
JournalJournal of Thoracic Disease
Volume11
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea grant (NRF-2018R1D1A1A02049260) funded by the Ministry of Education, and by the Bio & Medical Technology Development Program (NRF- 2017M3A9E8033225) in South Korea.

Funding Information:
Funding: This study was supported by the National Research Foundation of Korea grant (NRF-2018R1D1A1A02049260) funded by the Ministry of Education, and by the Bio & Medical Technology Development Program (NRF-2017M3A9E8033225) in South Korea.

Publisher Copyright:
© 2019 Journal of Thoracic Disease. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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