Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay

Kyung Hee Park, Jongsun Lee, Sang Chul Lee, Young Woong Son, Da Woon Sim, Jae Hyun Lee, Jung Won Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

Original languageEnglish
Pages (from-to)786-792
Number of pages7
JournalYonsei medical journal
Volume58
Issue number4
DOIs
Publication statusPublished - 2017 Jul

Fingerprint

Alternaria
Allergens
Immunoglobulin E
Dander
Ambrosia
Betula
Lolium
Egg White
Milk
Cats
Dermatophagoides farinae
Dogs
Dermatophagoides Antigens
Artemisia
Pyroglyphidae
Gold
Food
Serum
Arachis

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Park, Kyung Hee ; Lee, Jongsun ; Lee, Sang Chul ; Son, Young Woong ; Sim, Da Woon ; Lee, Jae Hyun ; Park, Jung Won. / Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay. In: Yonsei medical journal. 2017 ; Vol. 58, No. 4. pp. 786-792.
@article{e949feed37df42899acf2e3bf31bd85e,
title = "Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay",
abstract = "Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.",
author = "Park, {Kyung Hee} and Jongsun Lee and Lee, {Sang Chul} and Son, {Young Woong} and Sim, {Da Woon} and Lee, {Jae Hyun} and Park, {Jung Won}",
year = "2017",
month = "7",
doi = "10.3349/ymj.2017.58.4.786",
language = "English",
volume = "58",
pages = "786--792",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "4",

}

Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay. / Park, Kyung Hee; Lee, Jongsun; Lee, Sang Chul; Son, Young Woong; Sim, Da Woon; Lee, Jae Hyun; Park, Jung Won.

In: Yonsei medical journal, Vol. 58, No. 4, 07.2017, p. 786-792.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay

AU - Park, Kyung Hee

AU - Lee, Jongsun

AU - Lee, Sang Chul

AU - Son, Young Woong

AU - Sim, Da Woon

AU - Lee, Jae Hyun

AU - Park, Jung Won

PY - 2017/7

Y1 - 2017/7

N2 - Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

AB - Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=85019770841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019770841&partnerID=8YFLogxK

U2 - 10.3349/ymj.2017.58.4.786

DO - 10.3349/ymj.2017.58.4.786

M3 - Article

C2 - 28540992

AN - SCOPUS:85019770841

VL - 58

SP - 786

EP - 792

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 4

ER -