Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

Jeong Yeol Seo, Bing Chun Yan, Joon Ha Park, Ji Hyeon Ahn, In Hye Kim, Jae Chul Lee, Young Geun Kwon, Young Myeong Kim, Jun Hwi Cho, Moo Ho Won

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3 Citations (Scopus)

Abstract

Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalJournal of the Neurological Sciences
Volume325
Issue number1-2
DOIs
Publication statusPublished - 2013 Feb 15

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Hippocampal CA1 Region
Transient Ischemic Attack
N-Methyl-D-Aspartate Receptors
Young Adult
Ischemia
Gerbillinae
Reperfusion
Hippocampus
Pyramidal Cells
Maintenance
Proteins

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Seo, Jeong Yeol ; Yan, Bing Chun ; Park, Joon Ha ; Ahn, Ji Hyeon ; Kim, In Hye ; Lee, Jae Chul ; Kwon, Young Geun ; Kim, Young Myeong ; Cho, Jun Hwi ; Won, Moo Ho. / Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia. In: Journal of the Neurological Sciences. 2013 ; Vol. 325, No. 1-2. pp. 108-114.
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Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia. / Seo, Jeong Yeol; Yan, Bing Chun; Park, Joon Ha; Ahn, Ji Hyeon; Kim, In Hye; Lee, Jae Chul; Kwon, Young Geun; Kim, Young Myeong; Cho, Jun Hwi; Won, Moo Ho.

In: Journal of the Neurological Sciences, Vol. 325, No. 1-2, 15.02.2013, p. 108-114.

Research output: Contribution to journalArticle

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T1 - Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

AU - Seo, Jeong Yeol

AU - Yan, Bing Chun

AU - Park, Joon Ha

AU - Ahn, Ji Hyeon

AU - Kim, In Hye

AU - Lee, Jae Chul

AU - Kwon, Young Geun

AU - Kim, Young Myeong

AU - Cho, Jun Hwi

AU - Won, Moo Ho

PY - 2013/2/15

Y1 - 2013/2/15

N2 - Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.

AB - Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.

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