Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

Jeong Yeol Seo; Bing Chun Yan; Joon Ha Park; Ji Hyeon Ahn; In Hye Kim; Jae Chul Lee; Young Geun Kwon; Young Myeong Kim; Jun Hwi Cho; Moo Ho Won

doi:10.1016/j.jns.2012.12.012

Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

Jeong Yeol Seo, Bing Chun Yan, Joon Ha Park, Ji Hyeon Ahn, In Hye Kim, Jae Chul Lee, Young Geun Kwon, Young Myeong Kim, Jun Hwi Cho, Moo Ho Won

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6 Citations (Scopus)

Abstract

Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.

Original language	English
Pages (from-to)	108-114
Number of pages	7
Journal	Journal of the Neurological Sciences
Volume	325
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jns.2012.12.012
Publication status	Published - 2013 Feb 15

Bibliographical note

Funding Information:
The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012R1A1A2001404 ), and by a grant ( 2010K000823 ) from the Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.

All Science Journal Classification (ASJC) codes

Neurology
Clinical Neurology

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10.1016/j.jns.2012.12.012

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Seo, J. Y., Yan, B. C., Park, J. H., Ahn, J. H., Kim, I. H., Lee, J. C., Kwon, Y. G., Kim, Y. M., Cho, J. H., & Won, M. H. (2013). Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia. Journal of the Neurological Sciences, 325(1-2), 108-114. https://doi.org/10.1016/j.jns.2012.12.012

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title = "Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia",

abstract = "Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.",

author = "Seo, {Jeong Yeol} and Yan, {Bing Chun} and Park, {Joon Ha} and Ahn, {Ji Hyeon} and Kim, {In Hye} and Lee, {Jae Chul} and Kwon, {Young Geun} and Kim, {Young Myeong} and Cho, {Jun Hwi} and Won, {Moo Ho}",

note = "Funding Information: The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012R1A1A2001404 ), and by a grant ( 2010K000823 ) from the Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.",

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Seo, JY, Yan, BC, Park, JH, Ahn, JH, Kim, IH, Lee, JC, Kwon, YG, Kim, YM, Cho, JH & Won, MH 2013, 'Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia', Journal of the Neurological Sciences, vol. 325, no. 1-2, pp. 108-114. https://doi.org/10.1016/j.jns.2012.12.012

TY - JOUR

T1 - Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

AU - Seo, Jeong Yeol

AU - Yan, Bing Chun

AU - Park, Joon Ha

AU - Ahn, Ji Hyeon

AU - Kim, In Hye

AU - Lee, Jae Chul

AU - Kwon, Young Geun

AU - Kim, Young Myeong

AU - Cho, Jun Hwi

AU - Won, Moo Ho

N1 - Funding Information: The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012R1A1A2001404 ), and by a grant ( 2010K000823 ) from the Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.

PY - 2013/2/15

Y1 - 2013/2/15

N2 - Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.

AB - Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CA1) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CA1 region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1.

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Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

Abstract

Bibliographical note

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