Comparison of the oral microbiome of siblings using next-generation sequencing: a pilot study

H. S. Lee, J. H. Lee, S. O. Kim, JeSeon Song, Baekil Kim, Y. J. Kim, J. H. Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: The purpose of this study was to compare the oral microbiome of siblings with and without dental caries using next-generation sequencing. Materials and Methods: To investigate the oral microbiome composition, 14 young siblings, seven with caries and seven without, were enrolled from seven sibling-pair families. Supragingival plaque samples were collected from the cervicobuccal area of posterior teeth. All samples were analyzed by pyrosequencing, based on the 16S rRNA gene hypervariable regions, V1–V4. Results: The organisms identified belonged to 65 genera. Fifty-two genera were identified in the subjects with caries and 58 in those without; 45 genera were shared by both groups. In the Shannon index, the caries group showed lower bacterial diversity than the caries-free group and the difference was significant (Wilcoxon signed-rank test, P < 0.05). Additionally, similarities between siblings were evident in analyses based on weighted UniFrac distances (P < 0.05). Conclusions: In this study, the diversity of the microbiome was reduced in subjects with dental caries, while similarity between siblings seemed to be retained.

Original languageEnglish
Pages (from-to)549-556
Number of pages8
JournalOral Diseases
Volume22
Issue number6
DOIs
Publication statusPublished - 2016 Jan 1

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Microbiota
Dental Caries
Nonparametric Statistics
rRNA Genes
Tooth

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Lee, H. S. ; Lee, J. H. ; Kim, S. O. ; Song, JeSeon ; Kim, Baekil ; Kim, Y. J. ; Lee, J. H. / Comparison of the oral microbiome of siblings using next-generation sequencing : a pilot study. In: Oral Diseases. 2016 ; Vol. 22, No. 6. pp. 549-556.
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Comparison of the oral microbiome of siblings using next-generation sequencing : a pilot study. / Lee, H. S.; Lee, J. H.; Kim, S. O.; Song, JeSeon; Kim, Baekil; Kim, Y. J.; Lee, J. H.

In: Oral Diseases, Vol. 22, No. 6, 01.01.2016, p. 549-556.

Research output: Contribution to journalArticle

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