Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal

Mariane Martins de Araújo Stefani, Adriano Badotti Grassi, Lucas Henrique Sampaio, Ana Lúcia Osório Maroclo de Sousa, Maurício Barcelos Costa, Pauline Scheelbeek, Kapil D. Neupane, Deanna A. Hagge, Murdo Macdonald, Sangnae Cho, Linda Oskam, Samira Bührer-Sékula

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The diagnosis of leprosy continues to be based on clinical symptoms and early diagnosis and treatment are critical to preventing disability and transmission. Sensitive and specific laboratory tests are not available for diagnosing leprosy. Despite the limited applicability of anti-phenolic glycolipid-I (PGL-I) serology for diagnosis, it has been suggested as an additional tool to classify leprosy patients (LPs) for treatment purposes. Two formats of rapid tests to detect anti-PGL-I antibodies [ML immunochromatography assay (ICA) and ML Flow] were compared in different groups, multibacillary patients, paucibacillary patients, household contacts and healthy controls in Brazil and Nepal. High ML Flow intra-test concordance was observed and low to moderate agreement between the results of ML ICA and ML Flow tests on the serum of LPs was observed. LPs were "seroclassified" according to the results of these tests and the seroclassification was compared to other currently used classification systems: the World Health Organization operational classification, the bacilloscopic index and the Ridley-Jopling classification. When analysing the usefulness of these tests in the operational classification of PB and MB leprosy for treatment and follow-up purposes, the ML Flow test was the best point-of-care test for subjects in Nepal and despite the need for sample dilution, the ML ICA test yielded better performance among Brazilian subjects. Our results identified possible ways to improve the performance of both tests.

Original languageEnglish
Pages (from-to)124-131
Number of pages8
JournalMemorias do Instituto Oswaldo Cruz
Volume107
Issue numberSUPPL.1
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Nepal
Glycolipids
Leprosy
Serology
Brazil
Immunochromatography
Point-of-Care Systems
Early Diagnosis
Therapeutics
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Stefani, M. M. D. A., Grassi, A. B., Sampaio, L. H., de Sousa, A. L. O. M., Costa, M. B., Scheelbeek, P., ... Bührer-Sékula, S. (2012). Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal. Memorias do Instituto Oswaldo Cruz, 107(SUPPL.1), 124-131. https://doi.org/10.1590/S0074-02762012000900019
Stefani, Mariane Martins de Araújo ; Grassi, Adriano Badotti ; Sampaio, Lucas Henrique ; de Sousa, Ana Lúcia Osório Maroclo ; Costa, Maurício Barcelos ; Scheelbeek, Pauline ; Neupane, Kapil D. ; Hagge, Deanna A. ; Macdonald, Murdo ; Cho, Sangnae ; Oskam, Linda ; Bührer-Sékula, Samira. / Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal. In: Memorias do Instituto Oswaldo Cruz. 2012 ; Vol. 107, No. SUPPL.1. pp. 124-131.
@article{05725a8ccc6a4872922e7517b22646b9,
title = "Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal",
abstract = "The diagnosis of leprosy continues to be based on clinical symptoms and early diagnosis and treatment are critical to preventing disability and transmission. Sensitive and specific laboratory tests are not available for diagnosing leprosy. Despite the limited applicability of anti-phenolic glycolipid-I (PGL-I) serology for diagnosis, it has been suggested as an additional tool to classify leprosy patients (LPs) for treatment purposes. Two formats of rapid tests to detect anti-PGL-I antibodies [ML immunochromatography assay (ICA) and ML Flow] were compared in different groups, multibacillary patients, paucibacillary patients, household contacts and healthy controls in Brazil and Nepal. High ML Flow intra-test concordance was observed and low to moderate agreement between the results of ML ICA and ML Flow tests on the serum of LPs was observed. LPs were {"}seroclassified{"} according to the results of these tests and the seroclassification was compared to other currently used classification systems: the World Health Organization operational classification, the bacilloscopic index and the Ridley-Jopling classification. When analysing the usefulness of these tests in the operational classification of PB and MB leprosy for treatment and follow-up purposes, the ML Flow test was the best point-of-care test for subjects in Nepal and despite the need for sample dilution, the ML ICA test yielded better performance among Brazilian subjects. Our results identified possible ways to improve the performance of both tests.",
author = "Stefani, {Mariane Martins de Ara{\'u}jo} and Grassi, {Adriano Badotti} and Sampaio, {Lucas Henrique} and {de Sousa}, {Ana L{\'u}cia Os{\'o}rio Maroclo} and Costa, {Maur{\'i}cio Barcelos} and Pauline Scheelbeek and Neupane, {Kapil D.} and Hagge, {Deanna A.} and Murdo Macdonald and Sangnae Cho and Linda Oskam and Samira B{\"u}hrer-S{\'e}kula",
year = "2012",
month = "12",
day = "1",
doi = "10.1590/S0074-02762012000900019",
language = "English",
volume = "107",
pages = "124--131",
journal = "Memorias do Instituto Oswaldo Cruz",
issn = "0074-0276",
publisher = "Fundacao Oswaldo Cruz",
number = "SUPPL.1",

}

Stefani, MMDA, Grassi, AB, Sampaio, LH, de Sousa, ALOM, Costa, MB, Scheelbeek, P, Neupane, KD, Hagge, DA, Macdonald, M, Cho, S, Oskam, L & Bührer-Sékula, S 2012, 'Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal', Memorias do Instituto Oswaldo Cruz, vol. 107, no. SUPPL.1, pp. 124-131. https://doi.org/10.1590/S0074-02762012000900019

Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal. / Stefani, Mariane Martins de Araújo; Grassi, Adriano Badotti; Sampaio, Lucas Henrique; de Sousa, Ana Lúcia Osório Maroclo; Costa, Maurício Barcelos; Scheelbeek, Pauline; Neupane, Kapil D.; Hagge, Deanna A.; Macdonald, Murdo; Cho, Sangnae; Oskam, Linda; Bührer-Sékula, Samira.

In: Memorias do Instituto Oswaldo Cruz, Vol. 107, No. SUPPL.1, 01.12.2012, p. 124-131.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal

AU - Stefani, Mariane Martins de Araújo

AU - Grassi, Adriano Badotti

AU - Sampaio, Lucas Henrique

AU - de Sousa, Ana Lúcia Osório Maroclo

AU - Costa, Maurício Barcelos

AU - Scheelbeek, Pauline

AU - Neupane, Kapil D.

AU - Hagge, Deanna A.

AU - Macdonald, Murdo

AU - Cho, Sangnae

AU - Oskam, Linda

AU - Bührer-Sékula, Samira

PY - 2012/12/1

Y1 - 2012/12/1

N2 - The diagnosis of leprosy continues to be based on clinical symptoms and early diagnosis and treatment are critical to preventing disability and transmission. Sensitive and specific laboratory tests are not available for diagnosing leprosy. Despite the limited applicability of anti-phenolic glycolipid-I (PGL-I) serology for diagnosis, it has been suggested as an additional tool to classify leprosy patients (LPs) for treatment purposes. Two formats of rapid tests to detect anti-PGL-I antibodies [ML immunochromatography assay (ICA) and ML Flow] were compared in different groups, multibacillary patients, paucibacillary patients, household contacts and healthy controls in Brazil and Nepal. High ML Flow intra-test concordance was observed and low to moderate agreement between the results of ML ICA and ML Flow tests on the serum of LPs was observed. LPs were "seroclassified" according to the results of these tests and the seroclassification was compared to other currently used classification systems: the World Health Organization operational classification, the bacilloscopic index and the Ridley-Jopling classification. When analysing the usefulness of these tests in the operational classification of PB and MB leprosy for treatment and follow-up purposes, the ML Flow test was the best point-of-care test for subjects in Nepal and despite the need for sample dilution, the ML ICA test yielded better performance among Brazilian subjects. Our results identified possible ways to improve the performance of both tests.

AB - The diagnosis of leprosy continues to be based on clinical symptoms and early diagnosis and treatment are critical to preventing disability and transmission. Sensitive and specific laboratory tests are not available for diagnosing leprosy. Despite the limited applicability of anti-phenolic glycolipid-I (PGL-I) serology for diagnosis, it has been suggested as an additional tool to classify leprosy patients (LPs) for treatment purposes. Two formats of rapid tests to detect anti-PGL-I antibodies [ML immunochromatography assay (ICA) and ML Flow] were compared in different groups, multibacillary patients, paucibacillary patients, household contacts and healthy controls in Brazil and Nepal. High ML Flow intra-test concordance was observed and low to moderate agreement between the results of ML ICA and ML Flow tests on the serum of LPs was observed. LPs were "seroclassified" according to the results of these tests and the seroclassification was compared to other currently used classification systems: the World Health Organization operational classification, the bacilloscopic index and the Ridley-Jopling classification. When analysing the usefulness of these tests in the operational classification of PB and MB leprosy for treatment and follow-up purposes, the ML Flow test was the best point-of-care test for subjects in Nepal and despite the need for sample dilution, the ML ICA test yielded better performance among Brazilian subjects. Our results identified possible ways to improve the performance of both tests.

UR - http://www.scopus.com/inward/record.url?scp=84871518710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871518710&partnerID=8YFLogxK

U2 - 10.1590/S0074-02762012000900019

DO - 10.1590/S0074-02762012000900019

M3 - Article

VL - 107

SP - 124

EP - 131

JO - Memorias do Instituto Oswaldo Cruz

JF - Memorias do Instituto Oswaldo Cruz

SN - 0074-0276

IS - SUPPL.1

ER -

Stefani MMDA, Grassi AB, Sampaio LH, de Sousa ALOM, Costa MB, Scheelbeek P et al. Comparison of two rapid tests for anti-phenolic glycolipid-i serology in Brazil and Nepal. Memorias do Instituto Oswaldo Cruz. 2012 Dec 1;107(SUPPL.1):124-131. https://doi.org/10.1590/S0074-02762012000900019