Comparison of validity between WST-1 and MTT test in bioceramic materials

Kwang Mahn Kim, Sang Bae Lee, Se Ho Lee, Yong Keun Lee, Kyoung Nam Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. Tlie calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by 18010993-12:2002. The biocompatibilities of the extracts were measured by MTT and WST-1 assay and their pH were measured with pH meter. The cellular survival rate of CPG was the lowest and the results of the WST-1 test showed results similar to those of the MTT test. Thus, proliferation assays using WST-1 maybe conveniently and routinely applicable to pre-clinical evaluation of bioceramics.

Original languageEnglish
Pages (from-to)585-588
Number of pages4
JournalKey Engineering Materials
Volume284-286
Publication statusPublished - 2005 Dec 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Kim, K. M., Lee, S. B., Lee, S. H., Lee, Y. K., & Kim, K. N. (2005). Comparison of validity between WST-1 and MTT test in bioceramic materials. Key Engineering Materials, 284-286, 585-588.