Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Ji Hong Bong, Tae Hun Kim, Jaeyong Jung, Soo Jeong Lee, Jeong Soo Sung, Chang Kyu Lee, Min Jung Kang, Hyun Ok Kim, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

Original languageEnglish
Pages (from-to)100-108
Number of pages9
JournalBiochip Journal
Volume15
Issue number1
DOIs
Publication statusPublished - 2021 Mar

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111.

Publisher Copyright:
© 2021, The Korean BioChip Society.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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