Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111.
© 2021, The Korean BioChip Society.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Electrical and Electronic Engineering