TY - JOUR
T1 - Complete genome sequence of the bacteriophage YMC/09/04/R1988 MRSA BP
T2 - A lytic phage from a methicillin-resistant Staphylococcus aureus isolate
AU - Jeon, Jongsoo
AU - D'Souza, Roshan
AU - Hong, Sung Kuk
AU - Lee, Yangsoon
AU - Yong, Dongeun
AU - Choi, Jongrak
AU - Lee, Kyungwon
AU - Chong, Yunsop
N1 - Publisher Copyright:
© 2014 Federation of European Microbiological Societies.
PY - 2014
Y1 - 2014
N2 - Methicillin-resistant Staphylococcus aureus (MRSA) is an increasing cause of serious infection, both in the community and hospital settings. Despite sophisticated strategies and efforts, the antibiotic options for treating MRSA infection have been narrowed due to the limited number of newly developed antimicrobials. Herein, we analyze the completely sequenced genome of a novel virulent phage YMC/09/04/R1988 MRSA BP as a potential alternative anti-MRSA agent, which lysed clinical isolates from a patient admitted to the hospital due to hip disarticulation. The phage contains a linear double-stranded DNA genome of 44 459 bp in length, with 33.37% GC content, 62 predicted open reading frames (ORFs), and annotated functions of only 23 ORFs that are associated with structural assembly, host lysis, DNA replication, and modification. It showed a broad host range (17 of 30 strains) against MRSA strains in clinical isolates.
AB - Methicillin-resistant Staphylococcus aureus (MRSA) is an increasing cause of serious infection, both in the community and hospital settings. Despite sophisticated strategies and efforts, the antibiotic options for treating MRSA infection have been narrowed due to the limited number of newly developed antimicrobials. Herein, we analyze the completely sequenced genome of a novel virulent phage YMC/09/04/R1988 MRSA BP as a potential alternative anti-MRSA agent, which lysed clinical isolates from a patient admitted to the hospital due to hip disarticulation. The phage contains a linear double-stranded DNA genome of 44 459 bp in length, with 33.37% GC content, 62 predicted open reading frames (ORFs), and annotated functions of only 23 ORFs that are associated with structural assembly, host lysis, DNA replication, and modification. It showed a broad host range (17 of 30 strains) against MRSA strains in clinical isolates.
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U2 - 10.1111/1574-6968.12580
DO - 10.1111/1574-6968.12580
M3 - Article
AN - SCOPUS:84925868737
VL - 359
SP - 144
EP - 146
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -