Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages

Naoko Takeda-Okuda, Shuji Mizumoto, Zui Zhang, Soo-Ki Kim, Chi Ho Lee, Byong Tae Jeon, Yoshinao Z. Hosaka, Kenji Kadomatsu, Shuhei Yamada, Jun ichi Tamura

Research output: Contribution to journalArticle

Abstract

Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed. GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate. Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations. High O- and N-sulfation degrees of HS correspond to high ossification. Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.

Original languageEnglish
JournalGlycoconjugate Journal
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Antlers
Deer
Glycosaminoglycans
Heparitin Sulfate
heparitinsulfate lyase
Sulfates
Heparin Lyase
Chondroitin ABC Lyase
Tissue
Keratan Sulfate
Disaccharides
Hyaluronic Acid
Osteogenesis
dermatan sulfate chondroitin sulfate

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Takeda-Okuda, Naoko ; Mizumoto, Shuji ; Zhang, Zui ; Kim, Soo-Ki ; Lee, Chi Ho ; Jeon, Byong Tae ; Hosaka, Yoshinao Z. ; Kadomatsu, Kenji ; Yamada, Shuhei ; Tamura, Jun ichi. / Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages. In: Glycoconjugate Journal. 2019.
@article{7c62b13fc7ec4de39abef5bc5cbc0ca9,
title = "Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages",
abstract = "Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed. GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate. Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations. High O- and N-sulfation degrees of HS correspond to high ossification. Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.",
author = "Naoko Takeda-Okuda and Shuji Mizumoto and Zui Zhang and Soo-Ki Kim and Lee, {Chi Ho} and Jeon, {Byong Tae} and Hosaka, {Yoshinao Z.} and Kenji Kadomatsu and Shuhei Yamada and Tamura, {Jun ichi}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s10719-019-09859-1",
language = "English",
journal = "Glycoconjugate Journal",
issn = "0282-0080",
publisher = "Springer Netherlands",

}

Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages. / Takeda-Okuda, Naoko; Mizumoto, Shuji; Zhang, Zui; Kim, Soo-Ki; Lee, Chi Ho; Jeon, Byong Tae; Hosaka, Yoshinao Z.; Kadomatsu, Kenji; Yamada, Shuhei; Tamura, Jun ichi.

In: Glycoconjugate Journal, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages

AU - Takeda-Okuda, Naoko

AU - Mizumoto, Shuji

AU - Zhang, Zui

AU - Kim, Soo-Ki

AU - Lee, Chi Ho

AU - Jeon, Byong Tae

AU - Hosaka, Yoshinao Z.

AU - Kadomatsu, Kenji

AU - Yamada, Shuhei

AU - Tamura, Jun ichi

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed. GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate. Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations. High O- and N-sulfation degrees of HS correspond to high ossification. Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.

AB - Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed. GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate. Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations. High O- and N-sulfation degrees of HS correspond to high ossification. Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.

UR - http://www.scopus.com/inward/record.url?scp=85060625137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060625137&partnerID=8YFLogxK

U2 - 10.1007/s10719-019-09859-1

DO - 10.1007/s10719-019-09859-1

M3 - Article

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 0282-0080

ER -