Compression-induced expression of glycolysis genes in CAFs correlates with EMT and angiogenesis gene expression in breast cancer

Baek Gil Kim, Jin Sol Sung, Yeonsue Jang, Yoon Jin Cha, Suki Kang, Hyun Ho Han, Joo Hyun Lee, Nam Hoon Cho

Research output: Contribution to journalArticle

Abstract

Tumor growth increases compressive stress within a tissue, which is associated with solid tumor progression. However, very little is known about how compressive stress contributes to tumor progression. Here, we show that compressive stress induces glycolysis in human breast cancer associated fibroblast (CAF) cells and thereby contributes to the expression of epithelial to mesenchymal (EMT)- and angiogenesis-related genes in breast cancer cells. Lactate production was increased in compressed CAF cells, in a manner dependent on the expression of metabolic genes ENO2, HK2, and PFKFB3. Conditioned medium from compressed CAFs promoted the proliferation of breast cancer cells and the expression of EMT and/or angiogenesis-related genes. In patient tissues with high compressive stress, the expression of compression-induced metabolic genes was significantly and positively correlated with EMT and/or angiogenesis-related gene expression and metastasis size. These findings illustrate a mechanotransduction pathway involving stromal glycolysis that may be relevant also for other solid tumours.

Original languageEnglish
Article number313
JournalCommunications Biology
Volume2
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

glycolysis
Glycolysis
angiogenesis
Compressive stress
Gene expression
breast neoplasms
Tumors
Genes
Breast Neoplasms
Gene Expression
gene expression
neoplasms
Cells
Fibroblasts
Neoplasms
genes
fibroblasts
Tissue
Conditioned Culture Medium
Lactic Acid

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Cite this

Kim, Baek Gil ; Sung, Jin Sol ; Jang, Yeonsue ; Cha, Yoon Jin ; Kang, Suki ; Han, Hyun Ho ; Lee, Joo Hyun ; Cho, Nam Hoon. / Compression-induced expression of glycolysis genes in CAFs correlates with EMT and angiogenesis gene expression in breast cancer. In: Communications Biology. 2019 ; Vol. 2, No. 1.
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Compression-induced expression of glycolysis genes in CAFs correlates with EMT and angiogenesis gene expression in breast cancer. / Kim, Baek Gil; Sung, Jin Sol; Jang, Yeonsue; Cha, Yoon Jin; Kang, Suki; Han, Hyun Ho; Lee, Joo Hyun; Cho, Nam Hoon.

In: Communications Biology, Vol. 2, No. 1, 313, 01.12.2019.

Research output: Contribution to journalArticle

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