Computational design of binding proteins to EGFR domain II

Yoon Sup Choi, Soomin Yoon, Kyung Lock Kim, Jiho Yoo, Parkyong Song, Minsoo Kim, Young Eun Shin, Won Jun Yang, Jung Eun Noh, Hyun Soo Cho, Sanguk Kim, Junho Chung, Sung Ho Ryu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We developed a process to produce novel interactions between two previously unrelated proteins. This process selects protein scaffolds and designs protein interfaces that bind to a surface patch of interest on a target protein. Scaffolds with shapes complementary to the target surface patch were screened using an exhaustive computational search of the human proteome and optimized by directed evolution using phage display. This method was applied to successfully design scaffolds that bind to epidermal growth factor receptor (EGFR) domain II, the interface of EGFR dimerization, with high reactivity toward the target surface patch of EGFR domain II. One potential application of these tailor-made protein interactions is the development of therapeutic agents against specific protein targets.

Original languageEnglish
Article numbere92513
JournalPloS one
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 7

Fingerprint

Epidermal Growth Factor Receptor
binding proteins
Carrier Proteins
Scaffolds
Proteins
proteins
scaffolding proteins
dimerization
proteome
Bacteriophages
Dimerization
bacteriophages
Proteome
epidermal growth factor receptors
therapeutics
Display devices
methodology

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Choi, Y. S., Yoon, S., Kim, K. L., Yoo, J., Song, P., Kim, M., ... Ryu, S. H. (2014). Computational design of binding proteins to EGFR domain II. PloS one, 9(4), [e92513]. https://doi.org/10.1371/journal.pone.0092513
Choi, Yoon Sup ; Yoon, Soomin ; Kim, Kyung Lock ; Yoo, Jiho ; Song, Parkyong ; Kim, Minsoo ; Shin, Young Eun ; Yang, Won Jun ; Noh, Jung Eun ; Cho, Hyun Soo ; Kim, Sanguk ; Chung, Junho ; Ryu, Sung Ho. / Computational design of binding proteins to EGFR domain II. In: PloS one. 2014 ; Vol. 9, No. 4.
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Choi, YS, Yoon, S, Kim, KL, Yoo, J, Song, P, Kim, M, Shin, YE, Yang, WJ, Noh, JE, Cho, HS, Kim, S, Chung, J & Ryu, SH 2014, 'Computational design of binding proteins to EGFR domain II', PloS one, vol. 9, no. 4, e92513. https://doi.org/10.1371/journal.pone.0092513

Computational design of binding proteins to EGFR domain II. / Choi, Yoon Sup; Yoon, Soomin; Kim, Kyung Lock; Yoo, Jiho; Song, Parkyong; Kim, Minsoo; Shin, Young Eun; Yang, Won Jun; Noh, Jung Eun; Cho, Hyun Soo; Kim, Sanguk; Chung, Junho; Ryu, Sung Ho.

In: PloS one, Vol. 9, No. 4, e92513, 07.04.2014.

Research output: Contribution to journalArticle

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AU - Kim, Minsoo

AU - Shin, Young Eun

AU - Yang, Won Jun

AU - Noh, Jung Eun

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AU - Chung, Junho

AU - Ryu, Sung Ho

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Choi YS, Yoon S, Kim KL, Yoo J, Song P, Kim M et al. Computational design of binding proteins to EGFR domain II. PloS one. 2014 Apr 7;9(4). e92513. https://doi.org/10.1371/journal.pone.0092513