Computational design of binding proteins to EGFR domain II

Yoon Sup Choi, Soomin Yoon, Kyung Lock Kim, Jiho Yoo, Parkyong Song, Minsoo Kim, Young Eun Shin, Won Jun Yang, Jung Eun Noh, Hyun Soo Cho, Sanguk Kim, Junho Chung, Sung Ho Ryu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We developed a process to produce novel interactions between two previously unrelated proteins. This process selects protein scaffolds and designs protein interfaces that bind to a surface patch of interest on a target protein. Scaffolds with shapes complementary to the target surface patch were screened using an exhaustive computational search of the human proteome and optimized by directed evolution using phage display. This method was applied to successfully design scaffolds that bind to epidermal growth factor receptor (EGFR) domain II, the interface of EGFR dimerization, with high reactivity toward the target surface patch of EGFR domain II. One potential application of these tailor-made protein interactions is the development of therapeutic agents against specific protein targets.

Original languageEnglish
Article numbere92513
JournalPloS one
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 7

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Choi, Y. S., Yoon, S., Kim, K. L., Yoo, J., Song, P., Kim, M., Shin, Y. E., Yang, W. J., Noh, J. E., Cho, H. S., Kim, S., Chung, J., & Ryu, S. H. (2014). Computational design of binding proteins to EGFR domain II. PloS one, 9(4), [e92513]. https://doi.org/10.1371/journal.pone.0092513