Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system

Jong Ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have investigated a confocal fluorescence detection system for 3D cultured mammalian cells in a microfluidic cell culture analog system. For direct measurement of metabolic changes in the microfluidic cell culture systems using 3D cell cultures, we constructed a compact and portable confocal fluorescence detection system based on discrete optical components. The confocal detection system was designed to provide a depth scan in situ without lateral imaging for fast scanning and acquisition of average fluorescence variation associated with a large number of cells in 3D cultures of microfluidic devices. The results were consistent with the data obtained by a standard confocal microscope and suggest the potential for monitoring cell dynamics in 3D cell-based assays.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs
Publication statusPublished - 2010 May 10
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7568
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

Fingerprint

Microfluidics
Cell culture
cultured cells
Cultured Cells
Cell Culture Techniques
Fluorescence
Cells
Lab-On-A-Chip Devices
fluorescence
cells
Optical Devices
microfluidic devices
Assays
acquisition
Microscopes
Cell Count
microscopes
analogs
Scanning
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Choi, J. R., Sung, J. H., Shuler, M. L., & Kim, D. (2010). Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII [75680X] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568). https://doi.org/10.1117/12.841729
Choi, Jong Ryul ; Sung, Jong Hwan ; Shuler, Michael L. ; Kim, Donghyun. / Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Choi, JR, Sung, JH, Shuler, ML & Kim, D 2010, Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII., 75680X, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.841729

Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system. / Choi, Jong Ryul; Sung, Jong Hwan; Shuler, Michael L.; Kim, Donghyun.

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. 75680X (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Choi JR, Sung JH, Shuler ML, Kim D. Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. 75680X. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.841729