Confocal fluorescence detection of cell-based assays using a digital micro-mirror device

Jong Ryul Choi; Jong Hwan Sung; Michael L. Shuler; Donghyun Kim

doi:10.1117/12.841738

Confocal fluorescence detection of cell-based assays using a digital micro-mirror device

Jong Ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Original language	English
Title of host publication	Emerging Digital Micromirror Device Based Systems and Applications II
DOIs	https://doi.org/10.1117/12.841738
Publication status	Published - 2010
Event	Emerging Digital Micromirror Device Based Systems and Applications II - San Francisco, CA, United States Duration: 2010 Jan 27 → 2010 Jan 27

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7596
ISSN (Print)	0277-786X

Other

Other	Emerging Digital Micromirror Device Based Systems and Applications II
Country	United States
City	San Francisco, CA
Period	10/1/27 → 10/1/27

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.841738

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Cite this

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title = "Confocal fluorescence detection of cell-based assays using a digital micro-mirror device",

abstract = "We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.",

author = "Choi, {Jong Ryul} and Sung, {Jong Hwan} and Shuler, {Michael L.} and Donghyun Kim",

year = "2010",

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language = "English",

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Choi, JR, Sung, JH, Shuler, ML & Kim, D 2010, Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. in Emerging Digital Micromirror Device Based Systems and Applications II., 759608, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7596, Emerging Digital Micromirror Device Based Systems and Applications II, San Francisco, CA, United States, 10/1/27. https://doi.org/10.1117/12.841738

Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. / Choi, Jong Ryul; Sung, Jong Hwan; Shuler, Michael L.; Kim, Donghyun.

Emerging Digital Micromirror Device Based Systems and Applications II. 2010. 759608 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7596).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

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