Abstract
We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.
| Original language | English |
|---|---|
| Title of host publication | Emerging Digital Micromirror Device Based Systems and Applications II |
| Volume | 7596 |
| DOIs | |
| Publication status | Published - 2010 May 3 |
| Event | Emerging Digital Micromirror Device Based Systems and Applications II - San Francisco, CA, United States Duration: 2010 Jan 27 → 2010 Jan 27 |
Other
| Other | Emerging Digital Micromirror Device Based Systems and Applications II |
|---|---|
| Country | United States |
| City | San Francisco, CA |
| Period | 10/1/27 → 10/1/27 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering
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Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. / Choi, Jong Ryul; Sung, Jong Hwan; Shuler, Michael L.; Kim, Donghyun.
Emerging Digital Micromirror Device Based Systems and Applications II. Vol. 7596 2010. 759608.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Confocal fluorescence detection of cell-based assays using a digital micro-mirror device
AU - Choi, Jong Ryul
AU - Sung, Jong Hwan
AU - Shuler, Michael L.
AU - Kim, Donghyun
PY - 2010/5/3
Y1 - 2010/5/3
N2 - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.
AB - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.
UR - http://www.scopus.com/inward/record.url?scp=77951580009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951580009&partnerID=8YFLogxK
U2 - 10.1117/12.841738
DO - 10.1117/12.841738
M3 - Conference contribution
SN - 9780819479921
VL - 7596
BT - Emerging Digital Micromirror Device Based Systems and Applications II
ER -