Confocal fluorescence detection of cell-based assays using a digital micro-mirror device

Jong Ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Original languageEnglish
Title of host publicationEmerging Digital Micromirror Device Based Systems and Applications II
DOIs
Publication statusPublished - 2010
EventEmerging Digital Micromirror Device Based Systems and Applications II - San Francisco, CA, United States
Duration: 2010 Jan 272010 Jan 27

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7596
ISSN (Print)0277-786X

Other

OtherEmerging Digital Micromirror Device Based Systems and Applications II
CountryUnited States
CitySan Francisco, CA
Period10/1/2710/1/27

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Choi, J. R., Sung, J. H., Shuler, M. L., & Kim, D. (2010). Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. In Emerging Digital Micromirror Device Based Systems and Applications II [759608] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7596). https://doi.org/10.1117/12.841738