Confocal fluorescence detection of cell-based assays using a digital micro-mirror device

Jong Ryul Choi; Jong Hwan Sung; Michael L. Shuler; Donghyun Kim

doi:10.1117/12.841738

Confocal fluorescence detection of cell-based assays using a digital micro-mirror device

Jong Ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Original language	English
Title of host publication	Emerging Digital Micromirror Device Based Systems and Applications II
Volume	7596
DOIs	https://doi.org/10.1117/12.841738
Publication status	Published - 2010 May 3
Event	Emerging Digital Micromirror Device Based Systems and Applications II - San Francisco, CA, United States Duration: 2010 Jan 27 → 2010 Jan 27

Other

Other	Emerging Digital Micromirror Device Based Systems and Applications II
Country	United States
City	San Francisco, CA
Period	10/1/27 → 10/1/27

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All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

Choi, J. R., Sung, J. H., Shuler, M. L., & Kim, D. (2010). Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. In Emerging Digital Micromirror Device Based Systems and Applications II (Vol. 7596). [759608] https://doi.org/10.1117/12.841738

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abstract = "We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.",

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Choi, JR, Sung, JH, Shuler, ML & Kim, D 2010, Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. in Emerging Digital Micromirror Device Based Systems and Applications II. vol. 7596, 759608, Emerging Digital Micromirror Device Based Systems and Applications II, San Francisco, CA, United States, 10/1/27. https://doi.org/10.1117/12.841738

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AB - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

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Access to Document

10.1117/12.841738