TY - GEN
T1 - Confocal fluorescence detection of cell-based assays using a digital micro-mirror device
AU - Choi, Jong Ryul
AU - Sung, Jong Hwan
AU - Shuler, Michael L.
AU - Kim, Donghyun
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.
AB - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.
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U2 - 10.1117/12.841738
DO - 10.1117/12.841738
M3 - Conference contribution
AN - SCOPUS:77951580009
SN - 9780819479921
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Emerging Digital Micromirror Device Based Systems and Applications II
T2 - Emerging Digital Micromirror Device Based Systems and Applications II
Y2 - 27 January 2010 through 27 January 2010
ER -