Confocal fluorescence detection of cell-based assays using a digital micro-mirror device

Jong Ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Original languageEnglish
Title of host publicationEmerging Digital Micromirror Device Based Systems and Applications II
Volume7596
DOIs
Publication statusPublished - 2010 May 3
EventEmerging Digital Micromirror Device Based Systems and Applications II - San Francisco, CA, United States
Duration: 2010 Jan 272010 Jan 27

Other

OtherEmerging Digital Micromirror Device Based Systems and Applications II
CountryUnited States
CitySan Francisco, CA
Period10/1/2710/1/27

Fingerprint

Micromirror
Confocal
Fluorescence
Assays
Mirrors
mirrors
fluorescence
Cell
cells
Lateral
Light modulation
Cell culture
Microfluidics
Optical systems
Imaging systems
Cell Culture
light modulation
pinholes
Imaging System
Optical System

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Choi, J. R., Sung, J. H., Shuler, M. L., & Kim, D. (2010). Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. In Emerging Digital Micromirror Device Based Systems and Applications II (Vol. 7596). [759608] https://doi.org/10.1117/12.841738
Choi, Jong Ryul ; Sung, Jong Hwan ; Shuler, Michael L. ; Kim, Donghyun. / Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. Emerging Digital Micromirror Device Based Systems and Applications II. Vol. 7596 2010.
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Choi, JR, Sung, JH, Shuler, ML & Kim, D 2010, Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. in Emerging Digital Micromirror Device Based Systems and Applications II. vol. 7596, 759608, Emerging Digital Micromirror Device Based Systems and Applications II, San Francisco, CA, United States, 10/1/27. https://doi.org/10.1117/12.841738

Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. / Choi, Jong Ryul; Sung, Jong Hwan; Shuler, Michael L.; Kim, Donghyun.

Emerging Digital Micromirror Device Based Systems and Applications II. Vol. 7596 2010. 759608.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

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Choi JR, Sung JH, Shuler ML, Kim D. Confocal fluorescence detection of cell-based assays using a digital micro-mirror device. In Emerging Digital Micromirror Device Based Systems and Applications II. Vol. 7596. 2010. 759608 https://doi.org/10.1117/12.841738