Confocal fluorescence lifetime imaging microscopy based on a real-time sampling method

Yoon S. Bae, Dongsoo Lee, Sucbei Moon, Young Jae Won, Dug Y. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We present a fluorescence lifetime imaging microscope (FLIM) based on a real-time waveform acquisition method. The fluorophores were excited by a 635-nm gain-switched laser diode, which produced short pulses with duration ∼50 ps in a 20-MHz repetition rate. The fluorescence signals were detected by a silicon avalanche photo-diode (APD) in addition to a wide-band electric amplifier. The converted electric pulses were sampled by a high-speed digitizer of which sampling rate was 2 GS/s. In order to reduce the sampling interval for analyzing sub-nanosecond lifetimes, an interleaved data acquisition technique was used. The effective sampling rate was increased to 10 GS/s. In addition, the impulse response was measured simultaneously with the lifetime signals by an interleaving manner and was used in calibration of the system. By using these methods, accurate lifetime information was acquired in a short time less than 8 μs.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XIV
DOIs
Publication statusPublished - 2007
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6443
ISSN (Print)1605-7422

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/25

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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