Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21Cip1 and p27Kip1

Hyang Jee, Su Hyung Lee, Jun Won Park, Bo Ram Lee, KiTaek Nam, Dae Yong Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and p21Cip1 and p27Kip1 expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. G1 arrest, up-regulation of cell cycle-regulatory proteins p21Cip1 and p27Kip1 was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalBMB reports
Volume46
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

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Cell proliferation
Cell Cycle Checkpoints
Stomach Neoplasms
Stomach
Cell Cycle Proteins
Carcinogenesis
Cells
Cell Proliferation
Tissue
Connexins
Flow cytometry
Bromodeoxyuridine
Gap Junctions
Assays
Proteins
Real-Time Polymerase Chain Reaction
Cell Cycle
Flow Cytometry
Messenger RNA
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Jee, Hyang ; Lee, Su Hyung ; Park, Jun Won ; Lee, Bo Ram ; Nam, KiTaek ; Kim, Dae Yong. / Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21Cip1 and p27Kip1. In: BMB reports. 2013 ; Vol. 46, No. 1. pp. 25-30.
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Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21Cip1 and p27Kip1. / Jee, Hyang; Lee, Su Hyung; Park, Jun Won; Lee, Bo Ram; Nam, KiTaek; Kim, Dae Yong.

In: BMB reports, Vol. 46, No. 1, 01.01.2013, p. 25-30.

Research output: Contribution to journalArticle

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AU - Kim, Dae Yong

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