Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots

Eun Kyung Yoon, Souvik Dhar, Mi Hyun Lee, Jae Hyo Song, Shin Ae Lee, Gyuree Kim, Sejeong Jang, Ji Won Choi, Jeong Eun Choe, Jeong Hoe Kim, Myeong Min Lee, Jun Lim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.

Original languageEnglish
Pages (from-to)1197-1209
Number of pages13
JournalMolecular Plant
Volume9
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

Hypocotyl
endodermis
Arabidopsis
Starch
Molecular Biology
Transcription Factors
shoots
Proteins
molecular genetics
hypocotyls

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

Cite this

Yoon, Eun Kyung ; Dhar, Souvik ; Lee, Mi Hyun ; Song, Jae Hyo ; Lee, Shin Ae ; Kim, Gyuree ; Jang, Sejeong ; Choi, Ji Won ; Choe, Jeong Eun ; Kim, Jeong Hoe ; Lee, Myeong Min ; Lim, Jun. / Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots. In: Molecular Plant. 2016 ; Vol. 9, No. 8. pp. 1197-1209.
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title = "Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots",
abstract = "Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.",
author = "Yoon, {Eun Kyung} and Souvik Dhar and Lee, {Mi Hyun} and Song, {Jae Hyo} and Lee, {Shin Ae} and Gyuree Kim and Sejeong Jang and Choi, {Ji Won} and Choe, {Jeong Eun} and Kim, {Jeong Hoe} and Lee, {Myeong Min} and Jun Lim",
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Yoon, EK, Dhar, S, Lee, MH, Song, JH, Lee, SA, Kim, G, Jang, S, Choi, JW, Choe, JE, Kim, JH, Lee, MM & Lim, J 2016, 'Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots', Molecular Plant, vol. 9, no. 8, pp. 1197-1209. https://doi.org/10.1016/j.molp.2016.06.007

Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots. / Yoon, Eun Kyung; Dhar, Souvik; Lee, Mi Hyun; Song, Jae Hyo; Lee, Shin Ae; Kim, Gyuree; Jang, Sejeong; Choi, Ji Won; Choe, Jeong Eun; Kim, Jeong Hoe; Lee, Myeong Min; Lim, Jun.

In: Molecular Plant, Vol. 9, No. 8, 01.08.2016, p. 1197-1209.

Research output: Contribution to journalArticle

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T1 - Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots

AU - Yoon, Eun Kyung

AU - Dhar, Souvik

AU - Lee, Mi Hyun

AU - Song, Jae Hyo

AU - Lee, Shin Ae

AU - Kim, Gyuree

AU - Jang, Sejeong

AU - Choi, Ji Won

AU - Choe, Jeong Eun

AU - Kim, Jeong Hoe

AU - Lee, Myeong Min

AU - Lim, Jun

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.

AB - Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.

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