Abstract
Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.
| Original language | English |
|---|---|
| Pages (from-to) | 1197-1209 |
| Number of pages | 13 |
| Journal | Molecular Plant |
| Volume | 9 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2016 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Plant Science
Cite this
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Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots. / Yoon, Eun Kyung; Dhar, Souvik; Lee, Mi Hyun; Song, Jae Hyo; Lee, Shin Ae; Kim, Gyuree; Jang, Sejeong; Choi, Ji Won; Choe, Jeong Eun; Kim, Jeong Hoe; Lee, Myeong Min; Lim, Jun.
In: Molecular Plant, Vol. 9, No. 8, 01.08.2016, p. 1197-1209.Research output: Contribution to journal › Article
TY - JOUR
T1 - Conservation and Diversification of the SHR-SCR-SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots
AU - Yoon, Eun Kyung
AU - Dhar, Souvik
AU - Lee, Mi Hyun
AU - Song, Jae Hyo
AU - Lee, Shin Ae
AU - Kim, Gyuree
AU - Jang, Sejeong
AU - Choi, Ji Won
AU - Choe, Jeong Eun
AU - Kim, Jeong Hoe
AU - Lee, Myeong Min
AU - Lim, Jun
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.
AB - Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE 23 (SCL23). Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for specification of the endodermis (known as the bundle sheath) in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein complexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feedback loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morphological analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis (known as the starch sheath) in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.
UR - http://www.scopus.com/inward/record.url?scp=84991833303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991833303&partnerID=8YFLogxK
U2 - 10.1016/j.molp.2016.06.007
DO - 10.1016/j.molp.2016.06.007
M3 - Article
C2 - 27353361
AN - SCOPUS:84991833303
VL - 9
SP - 1197
EP - 1209
JO - Molecular Plant
JF - Molecular Plant
SN - 1674-2052
IS - 8
ER -