Constitutive GABA expression via a recombinant adeno-associated virus consistently attenuates neuropathic pain

Boyoung Lee, Jaehyung Kim, Sung Jin Kim, Heuiran Lee, Jin Woo Chang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Peripheral neuropathic pain is a common clinical problem with few existing treatments. Previously, we constructed rAAV bearing GAD65 and demonstrated that GAD65 and GABA can be constitutively produced in the CNS. To investigate the beneficial effects of GAD65 produced by rAAV and resulting GABA release in peripheral neuropathic pain, we established a neuropathic pain rat model. The direct administration of rAAV-GAD65 to dorsal root ganglion induced constitutive GAD65 expression, which was readily detected by immunohistochemistry. Both allodynic and hyperalgeic behavior tests suggested that neuropathic pain was noticeably reduced, along with the transgenic GAD65 expression. Moreover, the magnitude of pain relief was maintained during the entire experimental period. Concomitantly, the significant enhancement in GABA release following transgenic GAD65 expression was identified in vivo. Taken all together, these results provide evidence that persistent GAD65 and subsequent GABA expression in DRGs via rAAV effectively attenuates peripheral neuropathic pain for long period of time.

Original languageEnglish
Pages (from-to)971-976
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number4
DOIs
Publication statusPublished - 2007 Jun 15

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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