Construction of intragenic synthetic riboswitches for detection of a small molecule

Joon Jung Jo; Jong Shik Shin

doi:10.1007/s10529-009-0058-6

Construction of intragenic synthetic riboswitches for detection of a small molecule

Joon Jung Jo, Jong Shik Shin

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Bacterial sensors, based on ligand-mediated genetic control systems, are promising for on-site chemical detection because sensing targets and generating signals do not require costly instrumentation. Here, we have constructed intragenic synthetic riboswitches without relying on high-throughput screening and demonstrated that the riboswitches can be harnessed to develop bacterial sensors displaying readily visible reporter signals in response to theophylline. In vivo imaging using the riboswitch showed target-specific changes in the expression of a green fluorescence protein reporter, which was visible even to the naked eye.

Original language	English
Pages (from-to)	1577-1581
Number of pages	5
Journal	Biotechnology Letters
Volume	31
Issue number	10
DOIs	https://doi.org/10.1007/s10529-009-0058-6
Publication status	Published - 2009 Aug

Bibliographical note

Funding Information:
Acknowledgements Authors are grateful to Ms. S.-H. Ryu for the technical assistance to preliminary study. This work was supported by BK21 program from the Korean Ministry of Education, start-up research funds provided by Yonsei University and Seoul R&BD Program (NT080612, KU080657).

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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abstract = "Bacterial sensors, based on ligand-mediated genetic control systems, are promising for on-site chemical detection because sensing targets and generating signals do not require costly instrumentation. Here, we have constructed intragenic synthetic riboswitches without relying on high-throughput screening and demonstrated that the riboswitches can be harnessed to develop bacterial sensors displaying readily visible reporter signals in response to theophylline. In vivo imaging using the riboswitch showed target-specific changes in the expression of a green fluorescence protein reporter, which was visible even to the naked eye.",

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AB - Bacterial sensors, based on ligand-mediated genetic control systems, are promising for on-site chemical detection because sensing targets and generating signals do not require costly instrumentation. Here, we have constructed intragenic synthetic riboswitches without relying on high-throughput screening and demonstrated that the riboswitches can be harnessed to develop bacterial sensors displaying readily visible reporter signals in response to theophylline. In vivo imaging using the riboswitch showed target-specific changes in the expression of a green fluorescence protein reporter, which was visible even to the naked eye.

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Construction of intragenic synthetic riboswitches for detection of a small molecule

Abstract

Bibliographical note

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