Construction of luminescence- and fluorescence-tagged burkholderia pseudomallei for pathogen tracking in a mouse model

Yong Woo Shin, Deok Bum Park, Myung Min Choi, Jeong Hoon Chun, Baik Lin Seong, Gi Eun Rhie

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Molecular imaging is a powerful method for tracking various infectious disease-causing pathogens in host organisms. Currently, a dual molecular imaging method that can provide temporal and spatial information on infected hosts at the organism, organ, tissue, and cellular levels simultaneously has not been reported for Burkholderia pseudomallei, a high-risk pathogen that causes melioidosis. In this study, we have established an experimental method that provides spatiotemporal information on infected hosts using luminescent and fluorescent dual-labeled B. pseudomallei. Using this method, we visualized B. pseudomallei infection at the organism, organ, and tissue levels in a BALB/c mouse model by detecting its luminescence and fluorescence. The infection of B. pseudomallei at the cellular level was also visualized by its emitted fluorescence in infected macrophage cells. This method could be an extremely useful and applicable tool to study the pathogenesis of B. pseudomallei-related infectious diseases.

Original languageEnglish
Pages (from-to)498-502
Number of pages5
JournalJournal of microbiology and biotechnology
Volume28
Issue number3
DOIs
Publication statusPublished - 2018 Mar

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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