TY - JOUR
T1 - Construction of recombinant BCGs overexpressing antigen 85 complex and their protective efficacy against Mycobacterium tuberculosis infection in a mouse model
AU - Lee, Seung Heon
AU - Jeon, Bo Young
AU - Park, Young Gil
AU - Lee, Hye Young
AU - Cho, Sang Nae
AU - Kim, Hyo Joon
AU - Bai, Gill Han
PY - 2004/8
Y1 - 2004/8
N2 - Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
AB - Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
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U2 - 10.4046/trd.2004.57.2.125
DO - 10.4046/trd.2004.57.2.125
M3 - Article
AN - SCOPUS:7644240201
VL - 57
SP - 125
EP - 131
JO - Tuberculosis and Respiratory Diseases
JF - Tuberculosis and Respiratory Diseases
SN - 1738-3536
IS - 2
ER -
