Abstract
Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
| Original language | English |
|---|---|
| Pages (from-to) | 125-131 |
| Number of pages | 7 |
| Journal | Tuberculosis and Respiratory Diseases |
| Volume | 57 |
| Issue number | 2 |
| Publication status | Published - 2004 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Pulmonary and Respiratory Medicine
- Infectious Diseases
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Construction of recombinant BCGs overexpressing antigen 85 complex and their protective efficacy against Mycobacterium tuberculosis infection in a mouse model. / Lee, Seung Heon; Jeon, Bo Young; Park, Young Gil; Lee, Hyeyoung; Cho, Sangnae; Kim, Hyo Joon; Bai, Gill Han.
In: Tuberculosis and Respiratory Diseases, Vol. 57, No. 2, 01.08.2004, p. 125-131.Research output: Contribution to journal › Article
TY - JOUR
T1 - Construction of recombinant BCGs overexpressing antigen 85 complex and their protective efficacy against Mycobacterium tuberculosis infection in a mouse model
AU - Lee, Seung Heon
AU - Jeon, Bo Young
AU - Park, Young Gil
AU - Lee, Hyeyoung
AU - Cho, Sangnae
AU - Kim, Hyo Joon
AU - Bai, Gill Han
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
AB - Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
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M3 - Article
VL - 57
SP - 125
EP - 131
JO - Tuberculosis and Respiratory Diseases
JF - Tuberculosis and Respiratory Diseases
SN - 1738-3536
IS - 2
ER -