Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation

Hui Sung Moon, Kiho Kwon, Kyung A. Hyun, Tae Seok Sim, Jae Chan Park, Jeong Gun Lee, Hyo Il Jung

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer; as such, the ability to isolate and quantify CTCs is of great biomedical importance. This research presents a multi-stage multi-orifice flow fractionation (MS-MOFF) device formed by combining three single-stage multi-orifice segments designed for separating breast cancer cells from blood. The structure and dimensions of the MS-MOFF were determined by hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating non-selected target cells in comparison with the single-stage multi-orifice flow fractionation (SS-MOFF). The recovery of breast cancer cells increased from 88.8% to greater than 98.9% through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood, such as CTCs, in a label-free manner solely through the use of hydrodynamic forces.

Original languageEnglish
Article number014105
JournalBiomicrofluidics
Volume7
Issue number1
DOIs
Publication statusPublished - 2013 Feb 4

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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