Abstract
Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer; as such, the ability to isolate and quantify CTCs is of great biomedical importance. This research presents a multi-stage multi-orifice flow fractionation (MS-MOFF) device formed by combining three single-stage multi-orifice segments designed for separating breast cancer cells from blood. The structure and dimensions of the MS-MOFF were determined by hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating non-selected target cells in comparison with the single-stage multi-orifice flow fractionation (SS-MOFF). The recovery of breast cancer cells increased from 88.8% to greater than 98.9% through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood, such as CTCs, in a label-free manner solely through the use of hydrodynamic forces.
| Original language | English |
|---|---|
| Article number | 014105 |
| Journal | Biomicrofluidics |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 Feb 4 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Materials Science(all)
- Genetics
- Condensed Matter Physics
- Physical and Theoretical Chemistry
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Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation. / Moon, Hui Sung; Kwon, Kiho; Hyun, Kyung A.; Seok Sim, Tae; Chan Park, Jae; Lee, Jeong Gun; Jung, Hyo il.
In: Biomicrofluidics, Vol. 7, No. 1, 014105, 04.02.2013.Research output: Contribution to journal › Article
TY - JOUR
T1 - Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation
AU - Moon, Hui Sung
AU - Kwon, Kiho
AU - Hyun, Kyung A.
AU - Seok Sim, Tae
AU - Chan Park, Jae
AU - Lee, Jeong Gun
AU - Jung, Hyo il
PY - 2013/2/4
Y1 - 2013/2/4
N2 - Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer; as such, the ability to isolate and quantify CTCs is of great biomedical importance. This research presents a multi-stage multi-orifice flow fractionation (MS-MOFF) device formed by combining three single-stage multi-orifice segments designed for separating breast cancer cells from blood. The structure and dimensions of the MS-MOFF were determined by hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating non-selected target cells in comparison with the single-stage multi-orifice flow fractionation (SS-MOFF). The recovery of breast cancer cells increased from 88.8% to greater than 98.9% through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood, such as CTCs, in a label-free manner solely through the use of hydrodynamic forces.
AB - Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer; as such, the ability to isolate and quantify CTCs is of great biomedical importance. This research presents a multi-stage multi-orifice flow fractionation (MS-MOFF) device formed by combining three single-stage multi-orifice segments designed for separating breast cancer cells from blood. The structure and dimensions of the MS-MOFF were determined by hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating non-selected target cells in comparison with the single-stage multi-orifice flow fractionation (SS-MOFF). The recovery of breast cancer cells increased from 88.8% to greater than 98.9% through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood, such as CTCs, in a label-free manner solely through the use of hydrodynamic forces.
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U2 - 10.1063/1.4788914
DO - 10.1063/1.4788914
M3 - Article
VL - 7
JO - Biomicrofluidics
JF - Biomicrofluidics
SN - 1932-1058
IS - 1
M1 - 014105
ER -