Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation

Hui Sung Moon, Kiho Kwon, Kyung A. Hyun, Tae Seok Sim, Jae Chan Park, Jeong Gun Lee, Hyo il Jung

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer; as such, the ability to isolate and quantify CTCs is of great biomedical importance. This research presents a multi-stage multi-orifice flow fractionation (MS-MOFF) device formed by combining three single-stage multi-orifice segments designed for separating breast cancer cells from blood. The structure and dimensions of the MS-MOFF were determined by hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating non-selected target cells in comparison with the single-stage multi-orifice flow fractionation (SS-MOFF). The recovery of breast cancer cells increased from 88.8% to greater than 98.9% through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood, such as CTCs, in a label-free manner solely through the use of hydrodynamic forces.

Original languageEnglish
Article number014105
JournalBiomicrofluidics
Volume7
Issue number1
DOIs
Publication statusPublished - 2013 Feb 4

Fingerprint

orifice flow
Circulating Neoplastic Cells
orifices
Fractionation
Orifices
fractionation
blood
Tumors
Blood
tumors
cancer
Cells
Hydrodynamics
breast
Equipment and Supplies
Blood Cells
hydrodynamics
Breast Neoplasms
Aptitude
cells

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Moon, Hui Sung ; Kwon, Kiho ; Hyun, Kyung A. ; Seok Sim, Tae ; Chan Park, Jae ; Lee, Jeong Gun ; Jung, Hyo il. / Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation. In: Biomicrofluidics. 2013 ; Vol. 7, No. 1.
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Continual collection and re-separation of circulating tumor cells from blood using multi-stage multi-orifice flow fractionation. / Moon, Hui Sung; Kwon, Kiho; Hyun, Kyung A.; Seok Sim, Tae; Chan Park, Jae; Lee, Jeong Gun; Jung, Hyo il.

In: Biomicrofluidics, Vol. 7, No. 1, 014105, 04.02.2013.

Research output: Contribution to journalArticle

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