Continuous flow magnetic cell fractionation based on antigen expression level

Thomas Schneider, Lee R. Moore, Ying Jing, Seungjoo Haam, P. Stephen Williams, Aaron J. Fleischman, Shuvo Roy, Jeffrey J. Chalmers, Maciej Zborowski

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Cell separation is important in medical and biological research and plays an increasingly important role in clinical therapy and diagnostics, such as rare cancer cell detection in blood. The immunomagnetic labeling of cells with antibodies conjugated to magnetic nanospheres gives rise to a proportional relationship between the number of magnetic nanospheres attached to the cell and the cell surface marker number. This enables the potential fractionation of cell populations by magnetophoretic mobility (MM). We exploit this feature with our apparatus, the Dipole Magnet Flow Fractionator (DMFF), which consists of an isodynamic magnetic field, an orthogonally-oriented thin ribbon of cell suspension in continuous sheath flow, and ten outlet flows. From a sample containing a 1:1 mixture of immunomagnetically labeled (label+) and unlabeled (label-) cells, we achieved an increase in enrichment of the label+ cell fraction with increasing outlet numbers in the direction of the magnetic field gradient (up to 10-fold). The total recovery of the ten outlet fractions was 90.0 ± 7.7%. The mean MM of label+ cells increased with increasing outlet number by up to a factor of 2.3. The postulated proportionality between the number of attached magnetic beads and the number of cell surface markers was validated by comparison of MM measured by cell tracking velocimetry (CTV) with cell florescence intensity measured by flow cytometry.

Original languageEnglish
Pages (from-to)1-21
Number of pages21
JournalJournal of Biochemical and Biophysical Methods
Volume68
Issue number1
DOIs
Publication statusPublished - 2006 Jul 31

Bibliographical note

Funding Information:
The authors acknowledge the Dresden University of Technology, especially Prof. Th. Bley and Dr. E. Boschke for support of the work of Thomas Schneider. Supported by the U.S. Department of Defense (grant USAMRMC No.03051008) and the U.S. National Institutes of Health (R01 CA62349 and R01 CA97391). We also thankfully acknowledge technical assistance by Dr. Boris Kligman.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

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