Continuous labeling of circulating tumor cells with microbeads using a vortex micromixer for highly selective isolation

Ming Xian Lin, Kyung A. Hyun, Hui Sung Moon, Tae Seok Sim, Jeong Gun Lee, Jae Chan Park, Soo Suk Lee, Hyo Il Jung

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are identified in transit within the blood stream of cancer patients and have been proven to be a main cause of metastatic disease. Current approaches for the size-based isolation of CTCs have encountered technical challenges as some of the CTCs have a size similar to that of leukocytes and therefore CTCs are often lost in the process. Here, we propose a novel strategy where most of the CTCs are coated by a large number of microbeads to amplify their size to enable complete discrimination from leukocytes. In addition, all of the microbead labeling processes are carried out in a continuous manner to prevent any loss of CTCs during the isolation process. Thus, a microfluidic mixer was employed to facilitate the efficient and selective labeling of CTCs from peripheral blood samples. By generating secondary vortex flows called Taylor-Gortler vortices perpendicular to the main flow direction in our microfluidic device, CTCs were continuously and successfully coated with anti-epithelial cell adhesion molecule-conjugated beads. After the continuous labeling, the enlarged CTCs were perfectly trapped in a micro-filter whereas all of the leukocytes escaped.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalBiosensors and Bioelectronics
Volume40
Issue number1
DOIs
Publication statusPublished - 2013 Feb 15

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Circulating Neoplastic Cells
Microspheres
Labeling
Tumors
Vortex flow
Cells
Leukocytes
Lab-On-A-Chip Devices
Microfluidics
Blood
Cell Separation
Cell adhesion
Cell Adhesion Molecules
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Lin, Ming Xian ; Hyun, Kyung A. ; Moon, Hui Sung ; Sim, Tae Seok ; Lee, Jeong Gun ; Park, Jae Chan ; Lee, Soo Suk ; Jung, Hyo Il. / Continuous labeling of circulating tumor cells with microbeads using a vortex micromixer for highly selective isolation. In: Biosensors and Bioelectronics. 2013 ; Vol. 40, No. 1. pp. 63-67.
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Continuous labeling of circulating tumor cells with microbeads using a vortex micromixer for highly selective isolation. / Lin, Ming Xian; Hyun, Kyung A.; Moon, Hui Sung; Sim, Tae Seok; Lee, Jeong Gun; Park, Jae Chan; Lee, Soo Suk; Jung, Hyo Il.

In: Biosensors and Bioelectronics, Vol. 40, No. 1, 15.02.2013, p. 63-67.

Research output: Contribution to journalArticle

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