Continuous release of bFGF from multilayer nanofilm to maintain undifferentiated human iPS cell cultures

Ju Hyun Park; Jinkee Hong

doi:10.1039/c4ib00210e

Continuous release of bFGF from multilayer nanofilm to maintain undifferentiated human iPS cell cultures

Ju Hyun Park, Jinkee Hong

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In most cases, induced pluripotent stem (iPS) cells must be maintained by replacing basic fibroblast growth factor (bFGF)-containing medium daily to inhibit spontaneous differentiation. However, complete inhibition of differentiation is difficult to achieve even with frequent replacement of medium because of bFGF instability. Here, we established a novel system for the sustained release of bFGF from a modified surface by using a nanofilm fabrication method.

Original language	English
Pages (from-to)	1196-1200
Number of pages	5
Journal	Integrative Biology (United Kingdom)
Volume	6
Issue number	12
DOIs	https://doi.org/10.1039/c4ib00210e
Publication status	Published - 2014 Dec 1

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry

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10.1039/c4ib00210e

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abstract = "In most cases, induced pluripotent stem (iPS) cells must be maintained by replacing basic fibroblast growth factor (bFGF)-containing medium daily to inhibit spontaneous differentiation. However, complete inhibition of differentiation is difficult to achieve even with frequent replacement of medium because of bFGF instability. Here, we established a novel system for the sustained release of bFGF from a modified surface by using a nanofilm fabrication method.",

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N2 - In most cases, induced pluripotent stem (iPS) cells must be maintained by replacing basic fibroblast growth factor (bFGF)-containing medium daily to inhibit spontaneous differentiation. However, complete inhibition of differentiation is difficult to achieve even with frequent replacement of medium because of bFGF instability. Here, we established a novel system for the sustained release of bFGF from a modified surface by using a nanofilm fabrication method.

AB - In most cases, induced pluripotent stem (iPS) cells must be maintained by replacing basic fibroblast growth factor (bFGF)-containing medium daily to inhibit spontaneous differentiation. However, complete inhibition of differentiation is difficult to achieve even with frequent replacement of medium because of bFGF instability. Here, we established a novel system for the sustained release of bFGF from a modified surface by using a nanofilm fabrication method.

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Continuous release of bFGF from multilayer nanofilm to maintain undifferentiated human iPS cell cultures

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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