Contrast-enhanced ultrasonography for the evaluation of liver fibrosis after biliary obstruction

Hyun Joo Shin, Eun Young Chang, Hye Sun Lee, Jung Hwa Hong, Gyuri Park, Hyun Gi Kim, Myung Joon Kim, MiJung Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

AIM: To investigate perfusion change in contrast-enhanced ultrasonography (CEUS) to evaluate liver fibrosis based on biliary obstruction using an animal model. METHODS: New Zealand white rabbits (3-4 kg) underwent bile duct ligation to form a biliary obstruction model. We performed liver CEUS and laboratory tests on the day before the operation (day 0) and every 7 postoperative days until the rabbits were sacrificed. After CEUS, signal intensity of liver parenchyma with a time-intensity curve was analyzed. Perfusion parameters were automatically calculated from regionof-interests, including peak signal intensity, mean transit time, area under the curve and time to peak. Histological grades of liver fibrosis were assessed according to the Metavir score system immediately after sacrifice. Generalized estimating equations were used to analyze the association between liver fibrosis grades and perfusion parameters for statistical analysis. The perfusion parameters were measured on the last day and the difference between day 0 and the last day were evaluated. RESULTS: From the nine rabbits, histological grades of liver fibrosis were grade 1 in one rabbit, grade 2 and 3 in three rabbits each, and grade 4 in two rabbits. Among the four CEUS parameters, only the peak signal intensity measured on the last day demonstrated a significant association with liver fibrosis grades (OR = 1.392, 95%CI: 1.114-1.741, P = 0.004). The difference in peak signal intensity between day 0 and the last day also demonstrated an association with liver fibrosis (OR = 1.191, 95%CI: 0.999-1.419, P = 0.051). The other parameters tested, including mean transit time, area under the curve, and time to peak, showed no significant correlation with liver fibrosis grades. CONCLUSION: This animal study demonstrates that CEUS can be used to evaluate liver fibrosis from biliary obstruction using peak signal intensity as a parameter.

Original languageEnglish
Pages (from-to)2614-2621
Number of pages8
JournalWorld Journal of Gastroenterology
Volume21
Issue number9
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Liver Cirrhosis
Ultrasonography
Rabbits
Perfusion
Area Under Curve
Liver
Bile Ducts
Ligation
Animal Models

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Shin, Hyun Joo ; Chang, Eun Young ; Lee, Hye Sun ; Hong, Jung Hwa ; Park, Gyuri ; Kim, Hyun Gi ; Kim, Myung Joon ; Lee, MiJung. / Contrast-enhanced ultrasonography for the evaluation of liver fibrosis after biliary obstruction. In: World Journal of Gastroenterology. 2015 ; Vol. 21, No. 9. pp. 2614-2621.
@article{42485b0f7b2446a7a625c890a72845f2,
title = "Contrast-enhanced ultrasonography for the evaluation of liver fibrosis after biliary obstruction",
abstract = "AIM: To investigate perfusion change in contrast-enhanced ultrasonography (CEUS) to evaluate liver fibrosis based on biliary obstruction using an animal model. METHODS: New Zealand white rabbits (3-4 kg) underwent bile duct ligation to form a biliary obstruction model. We performed liver CEUS and laboratory tests on the day before the operation (day 0) and every 7 postoperative days until the rabbits were sacrificed. After CEUS, signal intensity of liver parenchyma with a time-intensity curve was analyzed. Perfusion parameters were automatically calculated from regionof-interests, including peak signal intensity, mean transit time, area under the curve and time to peak. Histological grades of liver fibrosis were assessed according to the Metavir score system immediately after sacrifice. Generalized estimating equations were used to analyze the association between liver fibrosis grades and perfusion parameters for statistical analysis. The perfusion parameters were measured on the last day and the difference between day 0 and the last day were evaluated. RESULTS: From the nine rabbits, histological grades of liver fibrosis were grade 1 in one rabbit, grade 2 and 3 in three rabbits each, and grade 4 in two rabbits. Among the four CEUS parameters, only the peak signal intensity measured on the last day demonstrated a significant association with liver fibrosis grades (OR = 1.392, 95{\%}CI: 1.114-1.741, P = 0.004). The difference in peak signal intensity between day 0 and the last day also demonstrated an association with liver fibrosis (OR = 1.191, 95{\%}CI: 0.999-1.419, P = 0.051). The other parameters tested, including mean transit time, area under the curve, and time to peak, showed no significant correlation with liver fibrosis grades. CONCLUSION: This animal study demonstrates that CEUS can be used to evaluate liver fibrosis from biliary obstruction using peak signal intensity as a parameter.",
author = "Shin, {Hyun Joo} and Chang, {Eun Young} and Lee, {Hye Sun} and Hong, {Jung Hwa} and Gyuri Park and Kim, {Hyun Gi} and Kim, {Myung Joon} and MiJung Lee",
year = "2015",
month = "1",
day = "1",
doi = "10.3748/wjg.v21.i9.2614",
language = "English",
volume = "21",
pages = "2614--2621",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "9",

}

Contrast-enhanced ultrasonography for the evaluation of liver fibrosis after biliary obstruction. / Shin, Hyun Joo; Chang, Eun Young; Lee, Hye Sun; Hong, Jung Hwa; Park, Gyuri; Kim, Hyun Gi; Kim, Myung Joon; Lee, MiJung.

In: World Journal of Gastroenterology, Vol. 21, No. 9, 01.01.2015, p. 2614-2621.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Contrast-enhanced ultrasonography for the evaluation of liver fibrosis after biliary obstruction

AU - Shin, Hyun Joo

AU - Chang, Eun Young

AU - Lee, Hye Sun

AU - Hong, Jung Hwa

AU - Park, Gyuri

AU - Kim, Hyun Gi

AU - Kim, Myung Joon

AU - Lee, MiJung

PY - 2015/1/1

Y1 - 2015/1/1

N2 - AIM: To investigate perfusion change in contrast-enhanced ultrasonography (CEUS) to evaluate liver fibrosis based on biliary obstruction using an animal model. METHODS: New Zealand white rabbits (3-4 kg) underwent bile duct ligation to form a biliary obstruction model. We performed liver CEUS and laboratory tests on the day before the operation (day 0) and every 7 postoperative days until the rabbits were sacrificed. After CEUS, signal intensity of liver parenchyma with a time-intensity curve was analyzed. Perfusion parameters were automatically calculated from regionof-interests, including peak signal intensity, mean transit time, area under the curve and time to peak. Histological grades of liver fibrosis were assessed according to the Metavir score system immediately after sacrifice. Generalized estimating equations were used to analyze the association between liver fibrosis grades and perfusion parameters for statistical analysis. The perfusion parameters were measured on the last day and the difference between day 0 and the last day were evaluated. RESULTS: From the nine rabbits, histological grades of liver fibrosis were grade 1 in one rabbit, grade 2 and 3 in three rabbits each, and grade 4 in two rabbits. Among the four CEUS parameters, only the peak signal intensity measured on the last day demonstrated a significant association with liver fibrosis grades (OR = 1.392, 95%CI: 1.114-1.741, P = 0.004). The difference in peak signal intensity between day 0 and the last day also demonstrated an association with liver fibrosis (OR = 1.191, 95%CI: 0.999-1.419, P = 0.051). The other parameters tested, including mean transit time, area under the curve, and time to peak, showed no significant correlation with liver fibrosis grades. CONCLUSION: This animal study demonstrates that CEUS can be used to evaluate liver fibrosis from biliary obstruction using peak signal intensity as a parameter.

AB - AIM: To investigate perfusion change in contrast-enhanced ultrasonography (CEUS) to evaluate liver fibrosis based on biliary obstruction using an animal model. METHODS: New Zealand white rabbits (3-4 kg) underwent bile duct ligation to form a biliary obstruction model. We performed liver CEUS and laboratory tests on the day before the operation (day 0) and every 7 postoperative days until the rabbits were sacrificed. After CEUS, signal intensity of liver parenchyma with a time-intensity curve was analyzed. Perfusion parameters were automatically calculated from regionof-interests, including peak signal intensity, mean transit time, area under the curve and time to peak. Histological grades of liver fibrosis were assessed according to the Metavir score system immediately after sacrifice. Generalized estimating equations were used to analyze the association between liver fibrosis grades and perfusion parameters for statistical analysis. The perfusion parameters were measured on the last day and the difference between day 0 and the last day were evaluated. RESULTS: From the nine rabbits, histological grades of liver fibrosis were grade 1 in one rabbit, grade 2 and 3 in three rabbits each, and grade 4 in two rabbits. Among the four CEUS parameters, only the peak signal intensity measured on the last day demonstrated a significant association with liver fibrosis grades (OR = 1.392, 95%CI: 1.114-1.741, P = 0.004). The difference in peak signal intensity between day 0 and the last day also demonstrated an association with liver fibrosis (OR = 1.191, 95%CI: 0.999-1.419, P = 0.051). The other parameters tested, including mean transit time, area under the curve, and time to peak, showed no significant correlation with liver fibrosis grades. CONCLUSION: This animal study demonstrates that CEUS can be used to evaluate liver fibrosis from biliary obstruction using peak signal intensity as a parameter.

UR - http://www.scopus.com/inward/record.url?scp=84925400654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925400654&partnerID=8YFLogxK

U2 - 10.3748/wjg.v21.i9.2614

DO - 10.3748/wjg.v21.i9.2614

M3 - Article

C2 - 25759528

AN - SCOPUS:84925400654

VL - 21

SP - 2614

EP - 2621

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 9

ER -